NIRS-Derived Muscle-Deoxygenation and Microvascular Reactivity During Occlusion-Reperfusion at Rest Are Associated With Whole-Body Aerobic Fitness

• Published in: Research quarterly for exercise and sport Vol. 95; no. 1; pp. 127 - 139
• Main Authors: Koutlas, Aggelos, Smilios, Ilias, Kokkinou, Eleni Maria, Myrkos, Aristides, Kounoupis, Anastasios, Dipla, Konstantina, Zafeiridis, Andreas
• Format: Journal Article
• Language: English
• Published: United States Routledge 02.01.2024
• Subjects: Maximal oxygen consumption; microvascular reactivity; muscle oxidative metabolism; near-infrared spectroscopy; microvascular reactivity; near-infrared spectroscopy; Maximal oxygen consumption; muscle oxidative metabolism
• ISSN: 0270-1367; 2168-3824; 2168-3824
• DOI: 10.1080/02701367.2022.2159309

Purpose: Near-infrared spectroscopy (NIRS) indices during arterial occlusion-reperfusion maneuver have been used to examine the muscle's oxidative metabolism and microvascular function-important determinants of whole-body aerobic-fitness. The association of NIRS-derived parameters with whole-body VO 2 max was previously examined using a method requiring exercise (or electrical stimulation) followed by multiple arterial occlusions. We examined whether NIRS-derived indices of muscle deoxygenation and microvascular reactivity assessed during a single occlusion-reperfusion at rest are (a) associated with maximal/submaximal indices of whole-body aerobic-fitness and (b) could discriminate individuals with different VO 2 max. We, also, investigated which NIRS-parameter during occlusion-reperfusion correlates best with whole-body aerobic-fitness. Methods: Twenty-five young individuals performed an arterial occlusion-reperfusion at rest. Changes in oxygenated- and deoxygenated-hemoglobin (O 2 Hb and HHb, respectively) in vastus-lateralis were monitored; adipose tissue thickness (ATT) at NIRS-application was assessed. Participants also underwent a maximal incremental exercise test for VO 2 max, maximal aerobic velocity (MAV), and ventilatory-thresholds (VTs) assessments. Results: The HHbslope and HHbmagnitude of increase (occlusion-phase) and O 2 Hbmagnitude of increase (reperfusion-phase) were strongly correlated with VO 2 max (r = .695-.763, p < .001) and moderately with MAV (r = .468-.530; p < .05). O 2 Hbmagnitude was moderately correlated with VTs (r = .399-.414; p < .05). After controlling for ATT, the correlations remained significant for VO 2 max (r = .672-.704; p < .001) and MAV (r = .407; p < .05). Individuals in the high percentiles after median and tritile splits for HHbslope and O 2 Hbmagnitude had significantly greater VO 2 max vs. those in low percentiles (p < .01-.05). The HHbslope during occlusion was the best predictor of VO 2 max. Conclusion: NIRS-derived muscle deoxygenation/reoxygenation indices during a single arterial occlusion-reperfusion maneuver are strongly associated with whole-body maximal indices of aerobic-fitness (VO 2 max, MAV) and may discriminate individuals with different VO 2 max.