Effect of swimming training on nerve morphological recovery after compressive injury

• Published in: Neurological research (New York) Vol. 40; no. 11; p. 955
• Main Authors: de Moraes, Alexa Alves, de Almeida, Cayo Antônio Soares, Lucas, Guilherme, Thomazini, José Antonio, DeMaman, Aline Santos
• Format: Journal Article
• Language: English
• Published: England 02.11.2018
• Subjects: Animals; Disease Models, Animal; Exercise Therapy; Male; Mice, Inbred BALB C; Nerve Compression Syndromes - pathology; Nerve Compression Syndromes - therapy; Nerve Degeneration - pathology; Nerve Degeneration - therapy; Nerve Regeneration; Neuralgia - pathology; Neuralgia - therapy; Random Allocation; Sciatic Nerve - pathology; Sciatic Neuropathy - pathology; Sciatic Neuropathy - therapy; Swimming; swimming; exercise; nerve regeneration; Neuropathic pain; sciatic nerve
• ISSN: 1743-1328
• DOI: 10.1080/01616412.2018.1504180

This study aims to investigate morphological alterations caused by partial sciatic nerve ligation (PNL) and the efficacy of a moderate-intensity swimming training as therapeutic strategy for nerve regeneration. A number of 30 male adult mice were equally divided in control, 14 days after PNL (PNL 14 days), 42 days after PNL (PNL 42 days), 70 days after PNL (PNL 70 days) and 5-week exercise training after 7 days post-lesion (PNL trained 35 days) groups. PNL trained 35 days group began with a 10-min session for 3 days and this time was gradually increased by 10 min every three sessions until the animals had swum for 50 min per session. Morphoquantitative analysis was carried out to assess nerve regeneration in each group. PNL 14 days group exhibited less degenerating signs than PNL 42 days group, where most post-lesion alterations were visualized. Nerve area and minimum diameter were significantly lower (p < 0.05) than control group. PNL 70 days group showed a greater degree of regenerating fibers and similar morphometric parameters to control group. PNL trained 35 days demonstrated signs of regeneration, reaching control group values in the morphometric analysis. PNL promotes great histopathological changes, which became more visible at 42 post-injury days. A natural nerve-regeneration tendency was observed throughout time, as observed in PNL 70 days group; nevertheless, moderate swimming training was found to be a therapeutic resource for nerve regeneration, accelerating such process from a morphoquantitative perspective. ANOVA: One-way analysis of variance; BDNF: Brain-derived neurotrophic factor; FGF-2: Fibroblast growth factor 2; GDNF: Glial cell line derived neurotrophic factor; IGF: Insulin-link growth factor; IL-1β: Interleukin-1β; NGF: Neural growth factor; PBS: Phosphate-buffered saline; PNL: Partial sciatic nerve ligation.