Platelet function and association of bovine viral diarrhea virus with platelets of persistently infected cattle

• Published in: American journal of veterinary research Vol. 66; no. 10; pp. 1738 - 1742
• Main Authors: Walz, P.H, Grooms, D.L, Bell, T.G, Kaiser, L, Baker, J.C, Brock, K.V
• Format: Journal Article
• Language: English
• Published: United States 01.10.2005
• Subjects: aggregometry; agonists; Animals; Blood Cell Count; blood platelet count; blood platelets; Blood Platelets - virology; blood serum; bovine viral diarrhea; Bovine viral diarrhea virus; Bovine Virus Diarrhea-Mucosal Disease - blood; Cattle; Diarrhea Virus 1, Bovine Viral - isolation & purification; Diarrhea Virus 1, Bovine Viral - metabolism; Diarrhea Virus 2, Bovine Viral - isolation & purification; Diarrhea Virus 2, Bovine Viral - metabolism; Female; host-pathogen relationships; Male; measurement; Platelet Activating Factor - pharmacology; Platelet Aggregation - drug effects; Platelet Aggregation - physiology; platelet-activating factor
• ISSN: 0002-9645; 1943-5681
• DOI: 10.2460/ajvr.2005.66.1738

To determine whether viral involvement with platelets obtained from cattle persistently infected (PI) with bovine viral diarrhea virus (BVDV) is associated with altered platelet function or decreased platelet counts. Platelets obtained from 8 cattle PI with BVDV and 6 age-, sex-, and breed-matched uninfected control cattle. Manual platelet counts were determined, and platelet function was assessed through optical aggregometry by use of the aggregation agonists ADP and platelet-activating factor. Identification of BVDV in serum and preparations of purified platelets was determined by use of virus isolation tests. No significant difference in platelet counts was detected between cattle PI with BVDV and control cattle. In response to the aggregation agonists, maximum aggregation percentage and slope of the aggregation curve were not significantly different between cattle PI with BVDV and control cattle. We isolated BVDV from serum of all PI cattle and from purified platelets of 6 of 8 PI cattle, but BVDV was not isolated from serum or platelets of control cattle. Isolation of BVDV from platelets in the peripheral circulation of cattle immunotolerant to BVDV does not result in altered platelet function or decreases in platelet counts.