MSTools—Web based application for visualization and presentation of HXMS data

• Published in: International journal of mass spectrometry Vol. 302; no. 1-3; pp. 53 - 58
• Main Authors: Kavan, Daniel, Man, Petr
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 30.04.2011
• Subjects: Data visualization; Hydrogen/deuterium exchange; Software; Web application; AA; Hydrogen/deuterium exchange; HXMS; Data visualization; Web application; Software; H/D; PDB
• ISSN: 1387-3806; 1873-2798
• DOI: 10.1016/j.ijms.2010.07.030

[Display omitted] ▶ MSTools are a web based platform for visualization of HXMS data. ▶ Several different modules aid in creation of various graphical outputs. ▶ Figures showing peptide map, plots for deuterium uptake over time, known 3D structure colored according to HXMS, or a heat map can be easily created using a simple text file. Hydrogen/deuterium exchange coupled to mass spectrometry is an excellent technique to study protein structure changes and interactions, especially in cases where traditional high-resolution structural techniques are not applicable. A major drawback of this technique is the absence of universal software tools for data processing and visualization of the results. Here we describe a set of PHP scripts facilitating H/D data visualization and presentation. The application runs on a web server and is freely accessible. It comprises tools helping in preparation of H/D experiments as well as tools for turning simple tables with data on H/D exchange into different ways of representation used by the HXMS community.