DNA typing using AmpFlSTR Y-filer for very long-term stored specimens

Abstract Aware that long-term storage bloodstain generally reduces numbers of detectable loci, we performed DNA typing with bloodstains stored at room temperature for 22–30 years, then extracted DNA from the bloodstains using the AmpFlSTR Y-filer PCR Amplification Kit. We performed electrophoresis w...

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Bibliographic Details
Published inLegal medicine (Tokyo, Japan) Vol. 11; pp. S466 - S467
Main Authors Tsukada, Kazuhiko, Shimizu, Maiko, Kurasawa, Yoshinobu, Kasahara, Koichi
Format Journal Article
LanguageEnglish
Published Ireland Elsevier Ireland Ltd 01.04.2009
Subjects
AmpFlSTR Y-filer
Blood Stains
Chromosomes, Human, Y
Degraded specimen
DNA - isolation & purification
DNA Degradation, Necrotic
DNA Fingerprinting
DNA typing
Electrophoresis, Agar Gel
Humans
Internal Medicine
Male
Old bloodstain
Polymerase Chain Reaction
Specimen Handling
Time Factors
Y-STR
Old bloodstain
Degraded specimen
Y-STR
DNA typing
AmpFlSTR Y-filer
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Summary:Abstract Aware that long-term storage bloodstain generally reduces numbers of detectable loci, we performed DNA typing with bloodstains stored at room temperature for 22–30 years, then extracted DNA from the bloodstains using the AmpFlSTR Y-filer PCR Amplification Kit. We performed electrophoresis with an ABI 310 Genetic Analyzer and determined alleles using GeneMapper ID v3.2 software. Our results suggest that Y-filer finds certain loci more difficult to anneal than others.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1344-6223
1873-4162
DOI:10.1016/j.legalmed.2009.01.057