Anti-adipogenic and lipid-lowering activity of piperine and epigallocatechin gallate in 3T3-L1 adipocytes

• Published in: Archives of physiology and biochemistry Vol. ahead-of-print; no. ahead-of-print; pp. 1 - 8
• Main Authors: Oruganti, Lokanatha, Reddy Sankaran, Karunakaran, Dinnupati, Haritha Goud, Kotakadi, Venkata Subbaiah, Meriga, Balaji
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 03.09.2023
• Subjects: 3T3-L1 cells; adipogenesis; epigallocatechin gallate; lipolysis; piperine; 3T3-L1 cells; piperine; adipogenesis; epigallocatechin gallate; lipolysis
• ISSN: 1381-3455; 1744-4160
• DOI: 10.1080/13813455.2021.1908366

The present study was aimed to evaluate the anti-adipogenic activity of piperine (PIP) and epigallocatechin gallate (EGCG) in 3T3-L1 cells. In cytotoxicity studies, PIP and EGCG showed IC 50 values of 260 and 218 µM respectively and in combination (20 µM each) did not show cytotoxicity. Treatment with PIP and EGCG (20 µM each) significantly (p<.01) inhibited cell differentiation, lipid droplets deposition and enhanced glycerol release in 3T3-L1 cells. The secreted level of leptin was decreased but adiponectin level was increased in treated 3T3-L1 cells than untreated cells. In molecular expression studies, key adipogenic genes PPAR-γ, SREBP-1c, FAS, Fab-4, C/EBP-α and HMG-CoA reductase were markedly down-regulated but UCP-1 was up-regulated in treated 3T3-L1 cells and the same trend was observed in expression levels of selected proteins. In conclusion, our results demonstrated a combination of PIP and EGCG exhibited strong anti-adipogenic and lipid lowering effect than individual treatments due to synergism.