The rat insulin-degrading enzyme: Molecular cloning and characterization of tissue-specific transcripts

• Published in: FEBS letters Vol. 317; no. 3; pp. 250 - 254
• Main Authors: Baumeister, Hans, Müller, Dieter, Rehbein, Monika, Richter, Dietmar
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.02.1993; Elsevier
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological and medical sciences; Blotting, Northern; brain; cDNA; Conserved Sequence; DNA - isolation & purification; Fundamental and applied biological sciences. Psychology; Gene expression; genes; Insulin-degrading enzyme; Insulysin - chemistry; Insulysin - genetics; Male; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; nucleotide sequence; olfactory bulb; Organ Specificity; Peroxisome; Polyadenylation site; predictions; Rats; RNA, Messenger - analysis; Testis; Testis - enzymology; Testis; Peroxisome; Polyadenylation site; Insulin-degrading enzyme; Rat; Nucleotide sequence; Enzyme; Rodentia; Gene expression; Testicle; Tissue; Vertebrata; Mammalia; Messenger RNA; Complementary DNA; Development
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(93)81286-9

The primary structure of the rat insulin-degrading enzyme (IDE) was determined by cDNA analysis. Rat IDE, as well as the previously characterized homologs from human and Drosophila, contain the carboxyl-terminal consensus sequence A/S-K-L for peroxisome targeting. A stretch of 43 bp surrounding an alternatively used polyadenylation site is highly conserved between rat and human, suggesting that it may contain important regulatory information. Northern blot analysis revealed two IDE transcripts of 3.7 and 5.5 kb in various tissues. Testis was found to be exceptional in having three different RNAs (3.7, 4.1 and 6.1 kb) at a relatively high abundance. The expression of the IDE gene in testis is correlated with sexual maturation.