Paclitaxel induces autophagy in gastric cancer BGC823 cells

• Published in: Ultrastructural pathology Vol. 41; no. 4; pp. 284 - 290
• Main Authors: Yu, Yi-Feng, Hu, Peng-Chao, Wang, Ying, Xu, Xiao-Long, Rushworth, Gerard Martin, Zhang, Zun, Wei, Lei, Zhang, Jing-Wei
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 04.07.2017
• Subjects: Antineoplastic Agents, Phytogenic - pharmacology; Autophagy; Autophagy - drug effects; BGC823; Cell Line, Tumor; Cell Survival - drug effects; Humans; paclitaxel; Paclitaxel - pharmacology; Stomach Neoplasms - pathology; ultrastructural changes; paclitaxel; BGC823; Autophagy; ultrastructural changes
• ISSN: 0191-3123; 1521-0758
• DOI: 10.1080/01913123.2017.1334019

This paper explores the connection between paclitaxel, a chemotherapeutic agent, and gastric cancer cells. In this experiment, it is demonstrated that paclitaxel triggers autophagy and inhibits proliferation of gastric cancer cells. An 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay was used to detect cell viability and the IC 50 of paclitaxel. Western blot was used to detect the expression levels of P62, and to measure the protein expression of autophagy. Immunofluorescence was used to reveal the appearance of punctate structures in the cytoplasm-this ultrastructure associated with autophagy was observed by microscopy. Electron microscopy revealed the formation of double-membrane autophagosomes, a typical structure of autophagy. In conclusion, our research indicates that paclitaxel may influence gastric cancer BGC823 cells by way of inducing autophagy.