There is no relation between the fascicular and the endoneural blood vessel areas on the cervical vagus nerve of normotensive rats

• Published in: Journal of neuroscience methods Vol. 158; no. 2; pp. 171 - 178
• Main Authors: Schiavoni, Maria Cristina Lopes, Fazan, Valéria Paula Sassoli
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.12.2006
• Subjects: Aging - physiology; Animals; Blood Vessels - anatomy & histology; Blood Vessels - growth & development; Blood Vessels - physiology; Body Weight - physiology; Cervical Vertebrae; Diet; Endoneural vessel; Fascicular area; Female; Functional Laterality - physiology; Linear Models; Morphometry; Nerve Fibers - physiology; Plastic Embedding; Rats; Rats, Inbred WKY; Regional Blood Flow - physiology; Vagus nerve; Vagus Nerve - anatomy & histology; Vagus Nerve - growth & development; Vagus Nerve - physiology; WKY rats; WKY rats; Vagus nerve; Endoneural vessel; Morphometry; Fascicular area
• ISSN: 0165-0270; 1872-678X
• DOI: 10.1016/j.jneumeth.2006.05.028

The aim of this study was to determine the existence of a relation between the fascicular area and total vessel area on the cervical vagus nerve of Wistar-Kyoto female rats. Animals aged 30, 180 or 360 days had their right vagus nerves prepared for epoxy resin embedding and light microscopy study. Semithin serial sections were studied every 100μm of the longitudinal extension of the nerve. The fascicular area and the total endoneural vessel area were obtained for each nerve segment (proximal, middle and distal) and compared between segments and ages. The relation between fascicular area and endoneural vessel area was accessed by linear regression analysis and correlation coefficient calculations. No significant relationship between the two variables was detected in all nerves studied. This suggests that the entrance of blood vessels to the endoneural space is not accompanied by an increase in the fascicular area and this may have implications on the fiber density calculations since, in the presence of large vessels, this density can be underestimated if the vessel area is not discounted. The endoneural vessel area increased from proximal to distal in all groups but got smaller with ageing, suggesting that older animals may be more susceptible to nerve ischemia.