Interleukin-12 inhibits cell invasion in choriocarcinoma
Gestational trophoblastic disease (GTD) is a unique disease that arises from allografting of the conceptus, and has a characteristic morphology and biological behavior. It encompasses a spectrum of interrelated diseases, including hydatidiform mole, invasive mole, choriocarcinoma and placental-site...
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Published in | International journal of molecular medicine Vol. 30; no. 1; pp. 57 - 62 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Greece
D.A. Spandidos
01.07.2012
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Subjects | |
Online Access | Get full text |
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Summary: | Gestational trophoblastic disease (GTD) is a unique disease that arises
from allografting of the conceptus, and has a characteristic morphology and biological
behavior. It encompasses a spectrum of interrelated diseases, including hydatidiform
mole, invasive mole, choriocarcinoma and placental-site trophoblastic tumor, but
its pathogenesis remains unrevealed. Particularly, choriocarcinoma is a highly
malignant tumor with poor prognosis. In this study, we cultured the human choriocarcinoma
cell line JEG-3 in vitro. After treating the cells with different doses of interleukin
(IL)-12, the cell invasion was observed. We also detected the expression of matrix
metalloproteinases (MMP)-9 and tissue inhibitors of metalloproteinases (TIMP)-1
and the cell cycle of JEG-3 cells. Our data indicated that IL-12 inhibits cell
invasion in a dose- and time-dependent manner through regulating the expression
of MMP-9 and TIMP-1. In addition, treatment with IL-12 redistributes the phases
of the cell cycle in JEG-3 cells. These findings suggest an antitumor role of
IL-12 in choriocarcinoma, with far reaching possibilities for understanding the
mechanisms of IL-12. |
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ISSN: | 1107-3756 1791-244X |
DOI: | 10.3892/ijmm.2012.960 |