Ligularia fischeri extract attenuates liver damage induced by chronic alcohol intake

• Published in: Pharmaceutical biology Vol. 54; no. 8; pp. 1465 - 1473
• Main Authors: Kim, Dongyeop, Kim, Gyeong-Woo, Lee, Seon-Ho, Han, Gi Dong
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 02.08.2016
• Subjects: Alcohol-fed rat model; alcoholic liver disease; Animals; Antioxidants - isolation & purification; Antioxidants - pharmacology; antioxidative enzyme; Asteraceae - chemistry; Biphenyl Compounds - chemistry; Cell Line; Cytochrome P-450 CYP2E1 - metabolism; Cytoprotection; Disease Models, Animal; Dose-Response Relationship, Drug; hepatoprotection; Lipid Peroxidation - drug effects; Liver - drug effects; Liver - metabolism; Liver - pathology; Liver Diseases, Alcoholic - metabolism; Liver Diseases, Alcoholic - pathology; Liver Diseases, Alcoholic - prevention & control; Male; Mice; Oxidative Stress - drug effects; Phytotherapy; Picrates - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Plant Leaves; Plants, Medicinal; Rats, Wistar; reactive oxygen species; Reactive Oxygen Species - metabolism; Superoxide Dismutase - metabolism; Tumor Necrosis Factor-alpha - metabolism; Alcohol-fed rat model; antioxidative enzyme; alcoholic liver disease; hepatoprotection; reactive oxygen species
• ISSN: 1388-0209; 1744-5116
• DOI: 10.3109/13880209.2015.1104701

Context Ligularia fischeri (Ledebour) Turcz. (Compositae) has been used as a leafy vegetable and in traditional medicine to treat hepatic disorder in East Asia. Objective The present study explores the antioxidant activity of LF aqueous extract on EtOH-induced oxidative stress accompanied by hepatotoxicity both in vitro and in vivo. Materials and methods In vitro study using the mouse liver NCTC-1469 cell line was conducted to estimate the cytotoxicity as well as the inhibitory effect of LF extract against alcohol-treated cell damage. In vivo study used an alcohol-fed Wister rat model orally administered EtOH (3.95 g/kg of body weight/d) with or without LF extract (100 or 200 mg/kg body weight) for 6 weeks. Serum and liver tissue were collected to evaluate hepatic injury and antioxidant-related enzyme activity. Results The EC 50 value for the DPPH radical scavenging capacity of LF extract was 451.5 μg/mL, whereas the IC 50 value of LF extract in terms of EtOH-induced reactive oxygen species (ROS) generation was 98.3 μg/mL without cell cytotoxicity. LF extract (200 mg/kg body weight) significantly reduced the triglyceride content of serum (33%) as well as hepatic lipid peroxidation (36%), whereas SOD activity was elevated three-fold. LF extract suppressed expression of CYP2E1 and TNF-α, and attenuated alcohol-induced abnormal morphological changes. Discussion and conclusion LF extract attenuated liver damage induced by alcoholic oxidative stress through inhibition of ROS generation, down-regulation of CYP2E1, and activation of hepatic antioxidative enzymes. Homeostasis of the antioxidative defence system in the liver by LF extract mitigated hepatic disorder following chronic alcohol intake.