Phylogenetic relationships of D. rudis (Bedriaga, 1886) and D. bithynica(Mehely, 1909) based on microsatellite and mitochondrial DNA in Turkey

The spiny-tailed lizard of the genus Darevskia have a series of taxonomic revisions, but still their phylogenetic relationships remain uncertain. In the present study, we have assessed taxonomic relationships among Darevskia bithynica and Darevskia rudis populations through estimation of phylogeneti...

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Published inMitochondrial DNA. Part A. DNA mapping, sequencing, and analysis Vol. 28; no. 6; pp. 814 - 825
Main Authors Koç, Halime, Kutrup, Bilal, Eroğlu, Oğuzhan, Bülbül, Ufuk, Kurnaz, Muammer, Afan, Feridun, Eroğlu, Ali İhsan
Format Journal Article
LanguageEnglish
Published England Taylor & Francis 02.11.2017
Subjects
16S rRNA
Animals
cytb
Cytochrome b Group - genetics
Genes, Mitochondrial
Genetic Variation
lizard
Lizards - genetics
Lizards - metabolism
microsatellite loci
Microsatellite Repeats
Phylogeny
polytomy
RNA, Ribosomal, 16S - genetics
Sequence Analysis, DNA
Turkey
microsatellite loci
16S rRNA
polytomy
cytb
lizard
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Summary:The spiny-tailed lizard of the genus Darevskia have a series of taxonomic revisions, but still their phylogenetic relationships remain uncertain. In the present study, we have assessed taxonomic relationships among Darevskia bithynica and Darevskia rudis populations through estimation of phylogenetic relationships among 96 specimens using microsatellite DNA (Du215, Du281, Du323 and Du418 loci) and 53 specimens using mtDNA (16S rRNA and cytb) from main populations in Turkey. Although D. b. bithynica and D. r. mirabilis were separated based on the PCoA analysis at low level from other D. rudis and D. bithynica populations, the distance values of Nei's genetic distance, Nei's unbiased genetic distance, Fst and Linear Fst were not high among taxa in microsatellite DNA. On the other hand, our phylogenetic analyses (NJ, ML, MP and BI) did not separate D. rudis and D. bithynica populations. Finally, most of the topologically identical trees of phylogenetic analyses and microsatellite results showed that the extant populations of D. rudis and D. bithynica were found to be polytomy. Based on our molecular phylogenetic study, D. rudis complex is still ongoing revisions.
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ISSN:2470-1394
2470-1408
DOI:10.1080/24701394.2016.1197215