Effect of tannic acid as crosslinking agent on fish skin gelatin-silver nanocomposite film

• Published in: Food packaging and shelf life Vol. 19; pp. 7 - 15
• Main Authors: Menezes, Maria do Livramento Linhares Rodrigues, Pires, Natália da Rocha, da Cunha, Pablyana Leila Rodrigues, de Freitas Rosa, Morsyleide, de Souza, Bartolomeu Warlene Silva, Feitosa, Judith Pessoa de Andrade, Souza Filho, Men de Sá Moreira de
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.03.2019
• Subjects: Antimicrobial activity; Gelatin; Nile tilapia; Silver nanoparticle; Tannic acid; Tannic acid; Antimicrobial activity; Nile tilapia; Gelatin; Silver nanoparticle
• ISSN: 2214-2894; 2214-2894
• DOI: 10.1016/j.fpsl.2018.11.005

[Display omitted] •Silver nanoparticles were synthesized and stabilized with Nile tilapia skin gelatin.•Tannic acid, a non-toxic phenol, was added as a cross-linker and co-reducing agent.•Films of gelatin, AgNPs, and tannic acid were strong and had a low water barrier.•Films showed antibacterial activities against S. aureus (gram+) and E. coli (gram-).•The films with tannic acid showed better properties to be used in food packaging. Gelatin was obtained from the Nile tilapia skin and used to prepare and stabilize silver nanoparticles (AgNPs) at a pH of 11.0. The properties gelatin-AgNP and gelatin-AgNP films with tannic acid (TA) were evaluated. TA is a non-toxic cross-linker agent and was add to enhance the mechanical properties of the gelatin-AgNPs nanocomposite. The films were studied by UV–vis and infrared (FT-IR) spectroscopy, thermal gravimetric analysis (TGA), mechanical analysis (tensile strength and elongation at break), water vapor permeability, solubility, scanning electron microscopy and antimicrobial activities (tested against two pathogens: S. aureus (gram-positive) and E. coli (gram-negative)). The gelatin films containing 5.0 wt% of TA, 0.2 wt% of AgNPs and 20 wt% of glycerol exhibited high mechanical strength, low water barrier, and antimicrobial activity against both pathogens. The presence of TA in gelatin-AgNPs nanocomposite was essential to promote these properties. Therefore, films with TA presented greater possibility to be used as food packaging.