Mapping of the RIB5 gene in Saccharomyces cerevisiae using UV light as an enhancer of rad52-mediated chromosome loss

Rib5 mutants of S. cerevisiae are blocked at the end of the riboflavin biosynthetic pathway. Using UV light to increase rad52-mediated chromosome loss, we have assigned the rib5 mutation to chromosome II. Tetrad analysis of crosses between rib5 and other markers on chromosome II shows that the RIB5...

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Bibliographic Details
Published inCurrent genetics Vol. 14; no. 5; pp. 419 - 423
Main Authors Angeles Santos, M. de los, Iturriaga, E.A, Eslava, A.P
Format Journal Article
LanguageEnglish
Published United States 01.11.1988
Subjects
biochemical pathways
chromosome ii
chromosome mapping
Chromosome Mapping - methods
Chromosomes - radiation effects
Crosses, Genetic
Diploidy
genes
Genes, Fungal
Genetic Markers
mutants
Mutation
regulation
riboflavin
Riboflavin - genetics
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - radiation effects
Ultraviolet Rays
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Summary:Rib5 mutants of S. cerevisiae are blocked at the end of the riboflavin biosynthetic pathway. Using UV light to increase rad52-mediated chromosome loss, we have assigned the rib5 mutation to chromosome II. Tetrad analysis of crosses between rib5 and other markers on chromosome II shows that the RIB5 gene is located on the right arm of this chromosome, closely linked to HIS7.
ISSN:0172-8083
1432-0983
DOI:10.1007/BF00521263