Immobilization of serum albumin on the synthesized three layers core–shell structures of super-paramagnetic iron oxide nanoparticles

• Published in: Journal of industrial and engineering chemistry (Seoul, Korea) Vol. 19; no. 5; pp. 1642 - 1647
• Main Authors: Khatiri, R., Reyhani, A., Mortazavi, S.Z., Hossainalipour, M.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 25.09.2013; 한국공업화학회
• Subjects: Albumin; APTES; Core-shell structure; Immobilization; Magnetic nanoparticles; Magnetite; Nanomaterials; Nanoparticles; Nanostructure; PEG; Polyethylene glycol; Serum albumin; Silanization; Silicon dioxide; 화학공학; Immobilization; Albumin; Silanization; APTES; PEG; Magnetic nanoparticles
• ISSN: 1226-086X; 1876-794X
• DOI: 10.1016/j.jiec.2013.02.002

In this study, the synthesis of four layer structures coated on magnetite nanoparticles such that Fe3O4/SiO2/APTES/PEG/BSA was investigated. Magnetite nanoparticles were synthesized by co-precipitating Fe2+ and Fe3+ in an ammonia solution with the average size of 25nm. To fabricate Fe3O4/SiO2 core–shells, the magnetite nanoparticles coated by silica with Stöber method. The fabricated nanoparticles were treated by 3-aminopropyl-triethoxysilane to achieve Fe3O4/SiO2/APTES nanostructures. Moreover, the nanoparticles were also attached to reactive polyethylene glycol chains. Eventually, the nanoparticles activated with bovine serum albumin for bio-application. X-ray diffraction, transmission electron microscopy, Fourier transform infrared spectroscopy, UV–vis spectroscopy and vibrating sample magnetometer were used to support the characterization.