Ag-MXene as peroxidase-mimicking nanozyme for enhanced bacteriocide and cholesterol sensing

• Published in: Journal of colloid and interface science Vol. 653; no. Pt A; pp. 540 - 550
• Main Authors: Chen, Yu, Rong, Chengyu, Gao, Wenhui, Luo, Siyu, Guo, Yuxin, Gu, Yingqiu, Yang, Guohai, Xu, Weiqing, Zhu, Chengzhou, Qu, Lu-Lu
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 01.01.2024
• Subjects: Ag-MXene; Antibacterial agents; biocides; biofilm; Biosensing; biosensors; blood serum; Cholesterol; cholesterol oxidase; colorimetry; detection limit; Nanozymes; rapid methods; reactive oxygen species; Nanozymes; Ag-MXene; Antibacterial agents; Biosensing; Cholesterol
• ISSN: 0021-9797; 1095-7103; 1095-7103
• DOI: 10.1016/j.jcis.2023.09.097

[Display omitted] •The prepared Ag-MXene artificial nanozyme showed high peroxidase-like activity.•The nanocomposite could enhance the antibacterial activity, and effectively disrupt the bacterial biofilm formation as well.•The peroxidase-like activity of Ag-MXene was coupled with cholesterol oxidase to realize the cholesterol detection.•The total cholesterol content in human serum samples have been successfully determined with satisfactory accuracy. Reactive oxygen species (ROS) are ideal alternative antibacterial reagents for rapid and effective sterilization. Although a variety of ROS-based antimicrobial strategies have been developed, many are still limited by their inefficiency. Herein, we report the synthesis of the Ag-MXene nanozyme, which have superior peroxidase-like activity for antibacterial applications. As a result, Ag-MXene nanozyme can efficiently increase the level of intracellular ROS, converting H2O2 into hydroxyl radicals that effectively kill both Gram-negative and Gram-positive bacteria and disrupting the bacterial biofilm formation. Moreover, a sensitive and selective colorimetric biosensor was constructed for assaying cholesterol based on the Ag-MXene’s prominent peroxidase-mimicking activity and the cholesterol oxidase cascade reaction. The biosensor exhibits high performance with a linear cholesterol detection range of 2–800 μM, and a detection limit of 0.6 μM. Ag-MXene nanozyme can be used for the rapid detection of cholesterol in serum without complicated sample pretreatment. Collectively, it is conceivable that the proposed Ag-MXene nanozyme could be used as a biocide and as a cholesterol sensor. This study provides a broad prospect for the rapid detection and sterilization of MXene nanozymes in the biomedical field.