The α and β thyroid receptors are expressed by cultured ependymal cells. Correlation with the effect of l-3,5,3′-triiodothyronine on glutamine synthetase mRNAs

• Published in: Neuroscience letters Vol. 150; no. 2; pp. 174 - 178
• Main Authors: Graff, M.N., Baas, D., Puymirat, J., Sarlieve, L.L., Delaunoy, J.P.
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 19.02.1993; Elsevier
• Subjects: Animals; Animals, Newborn - physiology; Anti-glutamine synthetase antibody; Anti-triiodothyronine receptor antibody; Autoradiography; Biochemistry, Molecular Biology; Biological and medical sciences; Cells, Cultured; Ependyma - metabolism; Fundamental and applied biological sciences. Psychology; Glutamate-Ammonia Ligase - biosynthesis; Glutamate-Ammonia Ligase - immunology; Immunocytochemistry; Immunohistochemistry; Isolated neuron and nerve. Neuroglia; Life Sciences; Primary ependymal cell culture; Rats; Rats, Wistar; Receptors, Thyroid Hormone - drug effects; Receptors, Thyroid Hormone - metabolism; RNA, Messenger - biosynthesis; Triiodothyronine - pharmacology; Up-Regulation - physiology; Vertebrates: nervous system and sense organs; Anti-glutamine synthetase antibody; Primary ependymal cell culture; Anti-triiodothyronine receptor antibody; Immunocytochemistry; Ependymal organ; Cell culture; Rat; Enzyme; Rodentia; Central nervous system; Beta-Peptide chain; Glutamine synthetase; Gene expression; Ependymocyte; Vertebrata; Mammalia; Thyroid hormone; Alpha-Peptide chain; Triiodothyronine; Brain (vertebrata); Hormonal receptor
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/0304-3940(93)90529-T

It is generally accepted that l-3,5,3′-triiodothyronine (L-T3) acts at the genomic level through an interaction with specific nuclear L-T3 receptors (NT 3R). Using antibodies raised against different peptides of NT 3R, we report here the immunocytochemical localization of the α, α2, β1 NT 3R subtypes in ependymal cell primary cultures. The α and β thyroid hormone receptors are both expressed. While the α and α2 subtypes are found in almost all cells, the β1 receptors are present in few cells only. The possibility that α and β receptors are colocalized is discussed. We also demonstrate that ependymal cells respond to L-T3 with a marked increase of the expression of the glutamine synthetase messenger RNAs.