A new method of DNA preparation for pulsed-field gel electrophoresis analysis

We have developed a new, labour-saving method of preparation, handling and treatment of DNA-containing agarose plugs for pulsed-field gel electrophoresis (PFGE). A plastic mould in which plugs are formed and supported during DNA purification and digestion was designed and successfully tested in a pr...

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Bibliographic Details
Published inJournal of microbiological methods Vol. 59; no. 3; pp. 433 - 436
Main Authors Fiett, Janusz, Kucharczyk, Krzysztof, Gniadkowski, Marek
Format Journal Article
LanguageEnglish
Published Shannon Elsevier B.V 01.12.2004
Elsevier Science
Subjects
Action of physical and chemical agents
Biological and medical sciences
DNA preparation
DNA, Bacterial - isolation & purification
Electrophoresis, Agar Gel - methods
Electrophoresis, Gel, Pulsed-Field - methods
Fundamental and applied biological sciences. Psychology
Microbiology
Mycological methods and techniques used in mycology
Mycology
PFGE
Virology
PFGE
DNA preparation
Design
Pulsed field electrophoresis
Prototype
Purification
Treatment
DNA
Genotype
Method
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Summary:We have developed a new, labour-saving method of preparation, handling and treatment of DNA-containing agarose plugs for pulsed-field gel electrophoresis (PFGE). A plastic mould in which plugs are formed and supported during DNA purification and digestion was designed and successfully tested in a prototype device.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2004.08.017