Effects of microwave ablation on T-cell subsets and cytokines of patients with hepatocellular carcinoma

• Published in: Minimally invasive therapy and allied technologies Vol. 26; no. 4; pp. 207 - 211
• Main Authors: Zhang, Haiwen, Hou, Xiaowei, Cai, Hongjian, Zhuang, Xingjun
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 04.07.2017
• Subjects: Aged; Carcinoma, Hepatocellular - surgery; Catheter Ablation - methods; CD4-Positive T-Lymphocytes - metabolism; CD8-Positive T-Lymphocytes - metabolism; cytokines; Cytokines - metabolism; Female; Flow Cytometry; hepatocellular carcinoma; Humans; immunoregulation; Interleukins - metabolism; Killer Cells, Natural - metabolism; Liver Neoplasms - surgery; Male; Microwave ablation; Microwaves; Middle Aged; T-cell subsets; T-Lymphocyte Subsets - metabolism; T-Lymphocytes, Regulatory - metabolism; Microwave ablation; immunoregulation; cytokines; T-cell subsets; hepatocellular carcinoma
• ISSN: 1364-5706; 1365-2931; 1365-2931
• DOI: 10.1080/13645706.2017.1286356

Objectives: To investigate the effects of microwave ablation on T-lymphocyte subsets and cytokines in patients with hepatocellular carcinoma. Material and methods: Peripheral blood was collected from 45 patients with hepatocellular carcinoma treated by microwave ablation before treatment, one week and one month after treatment. T cells (CD3+, CD4+ and CD8+ cells), CD4+ CD25+ Tregs, and CD16+ CD56+ NK cells were analyzed by flow cytometry. Levels of cytokines (IL-2, IFN-γ, TNF-α, IL-12, IL-4, IL-6, IL-8, and IL-10) were determined by a Luminex 200 analyzer. Results: Compared with before treatment, CD3+ cells, CD4+ cells and IL-12 increased significantly at one month after the microwave ablation treatment, while IL-4, IL-10 decreased significantly. Conclusions: Microwave ablation could relieve the suppression of immune function caused by tumors, promote the deviation of Th2/Th1, and improve immune dysfunction in patients with hepatocellular carcinoma.