Bacteriophage lambda-mediated transposon mutagenesis of phytopathogenic and epiphytic Erwinia species is strain dependent

Using transformation and conjugal mobilization, plasmids carrying the lamB gene of Escherichia coli were transferred to a range of Erwinia strains. The resultant strains were infected with lambda 467, and kanamycin resistant transductants were screened for various mutant phenotypes including auxotro...

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Bibliographic Details
Published inMolecular & general genetics Vol. 218; no. 3; pp. 491 - 498
Main Authors Ellard, F.M, Cabello, A, Salmond, G.P.C
Format Journal Article
LanguageEnglish
Published Germany 01.09.1989
Subjects
Bacteriophage lambda
conjugal mobilization
DNA Transposable Elements
Erwinia
Erwinia - genetics
Escherichia coli
genes
kanamycin
mobilization
mutagenesis
mutants
Mutation
phenotype
Plasmids
resistance
Restriction Mapping
species
Species Specificity
transduction
Transduction, Genetic
transfer
transformation
Transformation, Genetic
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Summary:Using transformation and conjugal mobilization, plasmids carrying the lamB gene of Escherichia coli were transferred to a range of Erwinia strains. The resultant strains were infected with lambda 467, and kanamycin resistant transductants were screened for various mutant phenotypes including auxotrophy and altered extracellular enzyme activities. Reversion analysis suggested that most mutant phenotypes were due to Tn5 insertion. The applicability of the techniques was highly strain dependent. However a rapid and simple route to mutant isolation was obtained, which could allow the use of other lambda-related genetic techniques in several important species which, to date, have not been genetically manipulated.
ISSN:0026-8925
1432-1874
DOI:10.1007/BF00332415