A rapid procedure for cell colony hybridization using DNA probes

A rapid, direct method for screening single cell-derived colonies or foci is described. The method allows the screening of a large number of colonies or foci by nitrocellulose filter hybridization using DNA probes. This technique simplifies current screening procedures and is a reliable, rapid, and...

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Bibliographic Details
Published inAnalytical biochemistry Vol. 179; no. 2; pp. 217 - 221
Main Authors Avraham, Hava, Avraham, Shalom, Bernstein, Shelly C.
Format Journal Article
LanguageEnglish
Published San Diego, CA Elsevier Inc 01.06.1989
Elsevier
Subjects
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Cell Line
DNA
DNA Probes
Dna, deoxyribonucleoproteins
Fundamental and applied biological sciences. Psychology
hybridization analysis
Immunoblotting
Mice
Nucleic Acid Hybridization
Nucleic acids
Plasmids
probes
Transfection
Cell culture
Molecular hybridization
Rodentia
Plasmid
Screening
Vertebrata
Mammalia
Transfection
Mouse
DNA
Blotting assay
Molecular probe
Southern blotting
Fibroblast
Nucleic acid
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Summary:A rapid, direct method for screening single cell-derived colonies or foci is described. The method allows the screening of a large number of colonies or foci by nitrocellulose filter hybridization using DNA probes. This technique simplifies current screening procedures and is a reliable, rapid, and sensitive method for the selection of cell clones containing a desired transfected gene.
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ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(89)90118-8