Multiparameter flow cytometry analysis of leukocyte markers for diagnosis in preterm neonatal sepsis

Neonatal sepsis is an important public health concern worldwide due to its immediate lethality and long-term morbidity rates, Clinical evaluation and laboratory analyses are indispensable for diagnosis of neonatal sepsis. However, assessing multiple biomarkers in neonates is difficult due to limited...

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Published inThe journal of maternal-fetal & neonatal medicine Vol. 34; no. 14; p. 2323
Main Authors Vázquez Rodríguez, Stephania, Arriaga Pizano, Lourdes A, Laresgoiti Servitje, Estibalitz, Mancilla Ramirez, Javier, Peralta Méndez, Omar L, Villalobos Alcazar, Gisela, Granados Cepeda, Martha L, Hernandez Pelaez, Maria Graciela, Cordero Gonzalez, Guadalupe, Arizmendi Villanueva, Roberto, Cruz Ramírez, José Luis, Isibasi, Armando, Lopez Macias, Constantino, Flores Romo, Leopoldo, Jimenez Zamudio, Luis A, Cérbulo-Vázquez, Arturo
Format Journal Article
LanguageEnglish
Published England 18.07.2021
Subjects
Biomarkers
Flow Cytometry
Humans
Infant, Newborn
Monocytes
Neonatal Sepsis - diagnosis
Sepsis - diagnosis
flow cytometry
neonate
Diagnosis
markers
sepsis
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Summary:Neonatal sepsis is an important public health concern worldwide due to its immediate lethality and long-term morbidity rates, Clinical evaluation and laboratory analyses are indispensable for diagnosis of neonatal sepsis. However, assessing multiple biomarkers in neonates is difficult due to limited blood availability. The aim is to investigate if the neonatal sepsis in preterm could be identified by multiparameter analysis with flow cytometry. The expression of activation-related molecules was evaluated by flow cytometry in newborn with or without risk factors for sepsis. Our analysis revealed that several markers could be useful for sepsis diagnosis, such as CD45RA, CD45RO, or CD71 on T cells; HLA-DR on NKT or classic monocytes, and TREM-1 on non-classic monocytes or neutrophils. However, ROC analysis shows that the expression of CD45RO on T lymphocytes is the only useful biomarker for diagnosis of neonatal late-onset sepsis. Also, decision tree analyses showed that CD45RO plus CD27 could help differentiate the preterm septic neonates from those with risk factors. Our study shows a complementary and practical strategy for biomarker assessment in neonatal sepsis.
ISSN:1476-4954
DOI:10.1080/14767058.2019.1666100