Systematic screening of protein modifications in four kinases using affinity enrichment and mass spectrometry analysis with unrestrictive sequence alignment

• Published in: Analytica chimica acta Vol. 691; no. 1; pp. 62 - 67
• Main Authors: Zhang, Kai, Zhu, Yixin, He, Xiwen, Zhang, Yukui
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 08.04.2011; Elsevier
• Subjects: Affinity enrichment; Alignment; Amino Acid Sequence; Analytical chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, Affinity - methods; Chromatography, High Pressure Liquid - methods; Enrichment; Exact sciences and technology; Glutathione S-transferase tag; Glutathione Transferase - genetics; Glutathione Transferase - metabolism; Isomers; Kinase; Kinases; Mass spectrometry; Molecular Sequence Data; Nanostructure; Nanotechnology; Other chromatographic methods; Phosphorylation; Post-translational modifications; Protein Kinases - chemistry; Protein Kinases - genetics; Protein Kinases - metabolism; Protein Processing, Post-Translational; Proteins; Recombinant Fusion Proteins - chemistry; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae Proteins - chemistry; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Sequence Alignment; Spectrometric and optical methods; Strategy; Tandem Mass Spectrometry - methods; Affinity enrichment; Phosphorylation; Post-translational modifications; Kinase; Mass spectrometry; Glutathione S-transferase tag; Phosphates; Yeast; Non-specific serine/threonine protein kinase; Identification; Chemical enrichment; Isomer; Design; Target; Glutathione transferase; Limitation; Shift; Purification; Enzyme; Transferases; Sample; Sequence alignment; Liquid chromatography; Method; Protein; Substrate; Screening; Mass spectrometry MS/MS; Transfer; Affinity; Strategy; Regulation; ATP
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2011.02.036

Protein kinases transfer phosphate groups from ATP to substrate proteins, they are known to be involved in diverse cellular processes. They are also important therapeutic targets in pharmaceutical design. Previous studies indicated that multiple post-translational modifications (PTMs) exist in kinases in addition to phosphorylation, and these PTMs play an important role in regulating kinases activities. Nevertheless, a comprehensive analysis for PTMs of kinases is insufficient due to technical limitations, which prevent us from better understanding their functional regulation. Here, we have developed a novel strategy that combines glutathione S-transferase tag affinity enrichment with nano-liquid chromatography coupled with tandem mass spectrometry analysis and non-restrictive protein sequence alignment for identification of diverse PTMs in four yeast kinases. The method allows us to enrich and analyze the entire protein isomers and to minimize the loss of all isomers of protein sample during protein purification. In our study, nineteen phosphorylation sites and several other types of PTMs sites were localized in 4 protein kinases. In addition, we found that some interesting mass shifts can not match those of the known PTMs. It suggested the existence of some undescribed PTMs in the proteins. Accordingly, this study showed that the novel strategy holds a great potential for identification of full-spectrum PTMs in proteins. Our data serves as a stepping stone for future functional studies.