The preventative effect of of Ro5-4864 (peripheral benzodiazepine receptor agonist) on spinal epidural fibrosis after laminectomy in a rat model

• Published in: Neurological research (New York) Vol. 43; no. 12; p. 1107
• Main Authors: Keskin, Emrah, Can, Emine Yılmaz, Aydın, Hasan Ali, Işık, Emre, Özgen, Utku, Şimşek, Kenan, Cengil, Osman, Başar, Cansu, Kalaycı, Murat
• Format: Journal Article
• Language: English
• Published: England 02.12.2021
• Subjects: Animals; Benzodiazepinones - pharmacology; Disease Models, Animal; Epidural Space - pathology; Failed Back Surgery Syndrome - prevention & control; Female; Laminectomy - adverse effects; Rats; Rats, Wistar; Tissue Adhesions - prevention & control; Ro5-4864; failed back surgery syndrome; TSPO; peripheral benzodiazepine receptor; epidural fibrosis; laminectomy
• ISSN: 1743-1328
• DOI: 10.1080/01616412.2021.1949689

To investigate the histopathological effects of a peripheral benzodiazepine receptor agonist (Ro5-4864) on epidural fibrosis (EF) in an experimental study model (post-laminectomy) in rats. A total of 32 albino Wistar rats were randomly divided into four equal groups (n = 8). In Group 1, no treatment was applied after laminectomy (control group). In Group 2, hemostasis was achieved after Laminectomy, and the surgical procedure was terminated by placing a 2-mm absorbable gelatin sponge dipped in saline into the epidural space. In Group 3, low-dose (4 mg/kg) Ro5-4864 was administered 30 minutes before the surgery. In Group 4, high-dose (8 mg/kg) Ro5-4864 was administered 30 minutes before the surgery. A histopathological examination was performed to evaluate arachnoidal invasion and EF. Our data revealed the EF was significantly reduced in rats treated with high-dose Ro5-4864 (Group 4) compared to the control and saline-soaked Spongostan groups (p = 0.000 and p = 0.006, respectively). There was no significant difference between the groups treated with high- and low-dose Ro5-4864. Arachnoidal invasion was not seen in any of the rats in the high-dose R05-4864 group. However, the arachnoidal invasion results did not significantly differ between the study groups (p = 0.052 = 0.05). Our study showed that Ro5-4864 could be effective in reducing EF in rats after.