LncRNA ENSG00000254615 Modulates Proliferation and 5-FU Resistance by Regulating p21 and Cyclin D1 in Colorectal Cancer

• Published in: Cancer investigation Vol. 39; no. 9; pp. 696 - 710
• Main Authors: Fu, Yang, Huang, Runqing, Li, Jianxia, Xie, Xiaoyu, Deng, Yanhong
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 21.10.2021
• Subjects: 5-FU resistance; Animals; Antimetabolites, Antineoplastic - pharmacology; Cell Line, Tumor; Cell Proliferation - genetics; Colorectal cancer; Colorectal Neoplasms - drug therapy; Colorectal Neoplasms - genetics; Colorectal Neoplasms - metabolism; Cyclin D1 - genetics; Cyclin D1 - metabolism; Cyclin-Dependent Kinase Inhibitor p21 - genetics; Cyclin-Dependent Kinase Inhibitor p21 - metabolism; Drug Resistance, Neoplasm - genetics; Fluorouracil - pharmacology; Gene Expression Regulation, Neoplastic; HCT116 Cells; HEK293 Cells; Humans; lncRNA; Mice; Mice, Inbred BALB C; Mice, Nude; proliferation; RNA, Long Noncoding - genetics; Tumor Burden - drug effects; Tumor Burden - genetics; Xenograft Model Antitumor Assays - methods; lncRNA; 5-FU resistance; proliferation; Colorectal cancer
• ISSN: 0735-7907; 1532-4192
• DOI: 10.1080/07357907.2021.1923727

5-Fluorouracil (5-FU) resistance is an urgent problem of colorectal cancer (CRC) chemotherapy that needs to be resolved. To investigate 5-FU-associated lncRNAs for CRC might be of great significant. LncRNA ENSG00000254615 was detected by RNA-sequencing. ENSG00000254615 were detected highly expressed in 5-FU-sensitive CRC cells and tissue specimens, and inhibited cell proliferation and attenuated 5-FU resistance in vitro and in vivo. Furthermore, ENSG00000254615 participated in the regulation of p21 and Cyclin D1. Taken together, we proposed that ENSG00000254615 inhibits proliferation and attenuates 5-FU resistance of CRC by regulating p21 and Cyclin D1 expression.