Replication of measles virus in the human plasma cell leukemia-derived LICR-LON-HMy2 cell line

Measles virus is usually grown in human or monkey fibroblast cells. We now show that LICR-LON-HMy2 (LL2) cells, a human plasma cell leukemia-derived line which grows in suspension culture, will permissively support replication of measles virus to an extent achievable with Vero cells. Sodium dodecyl...

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Bibliographic Details
Published inJournal of virological methods Vol. 24; no. 3; pp. 313 - 320
Main Authors Ziola, Barry, Morhart, Michael, Gilbert, Lynn, Karvonen, Brenda, Chen, Xiao Ping
Format Journal Article
LanguageEnglish
Published London Elsevier B.V 01.06.1989
Amsterdam Elsevier
New York, NY
Subjects
Animals
Antibodies, Monoclonal - immunology
Biological and medical sciences
Cell Line - microbiology
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
Hemagglutinin
Hemagglutinins, Viral - biosynthesis
Human plasma cell
Humans
LL2 cell
Measles virus
Measles virus - growth & development
Microbiology
Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains
Suspension cell
Vero Cells
Viral Proteins - analysis
Viral Proteins - immunology
Virology
Virus Cultivation - methods
Virus production
Human plasma cell
Virus production
Suspension cell
Hemagglutinin
LL2 cell
Measles virus
Virus
Human
Plasma
Cell line
Virus multiplication
Leukemia
Morbillivirus
Paramyxoviridae
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Summary:Measles virus is usually grown in human or monkey fibroblast cells. We now show that LICR-LON-HMy2 (LL2) cells, a human plasma cell leukemia-derived line which grows in suspension culture, will permissively support replication of measles virus to an extent achievable with Vero cells. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis of measles virions produced by LL2 cells showed a polypeptide pattern typical of measles virus. As well, measles virus-infected LL2 cells, like infected Vero cells, were found to secrete large amounts of virus hemagglutinin, but not other virus proteins. We thus conclude that LL2 cells can be effectively used to produce milligram amounts of measles virus and that virus-clarified culture medium from measles virus-infected LL2 cells is a potential source for purifying virus hemagglutinin.
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ISSN:0166-0934
1879-0984
DOI:10.1016/0166-0934(89)90043-8