Isolation of low-copy-number sequences that neighbor satellite DNA in mammals

To investigate the role of satellite DNA in eukaryotic genomes, we isolated from an African green monkey ( Cercopithecus aethiops) genomic library cloned segments containing the previously described deca-satellite linked to low-copy-number genomic sequences. Three such clones were obtained. The low-...

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Bibliographic Details
Published inGene Vol. 50; no. 1; pp. 299 - 311
Main Authors Maresca, Antonella, Thayer, Ronald E., Guenet, Chantal, Singer, Maxine F.
Format Journal Article
LanguageEnglish
Published Lausanne Elsevier B.V 1986
Amsterdam Elsevier
New York, NY
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Summary:To investigate the role of satellite DNA in eukaryotic genomes, we isolated from an African green monkey ( Cercopithecus aethiops) genomic library cloned segments containing the previously described deca-satellite linked to low-copy-number genomic sequences. Three such clones were obtained. The low-copy-number sequences in the three clones do not cross-hybridize suggesting that they derive from different genomic loci. The structure of one of the clones, λAMkA, is described in detail. Subcloned segments containing the low-copy-number sequences from λAMkA anneal to monkey, human and mouse genomic DNA. The subcloned probes were used to select clones containing homologous sequences from a second, independent monkey library as well as from human and mouse genomic libraries. Several of the newly isolated monkey clones hybridized to probes containing the species-specific deca- and α-satellites, confirming the genomic association of the low-copy-number sequence in λAMkA with satellite DNA. Moreover, several of the human and mouse clones hybridized to species-specific human and mouse satellite DNAs, respectively. These experiments indicate that the low-copy-number sequence in λMkA and its association with satellite DNA is conserved in primates and rodents.
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ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(86)90334-3