Extending flow cytometry sample stability by freezing lysed whole blood for clinical monitoring of Treg

A major challenge for flow cytometry assays supporting clinical trials is postcollection sample stability. Here we present an approach that could mitigate the stability issue while preserving sample integrity and cellular markers, especially when enumerating rare populations such as Tregs. Stability...

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Bibliographic Details
Published inBioanalysis Vol. 12; no. 10; pp. 655 - 663
Main Authors Yu, Jing, Jasti, Susmita, Mason, Deanna, Hays, Amanda
Format Journal Article
LanguageEnglish
Published England Newlands Press Ltd 01.05.2020
Newlands Press
Subjects
Anticoagulants
Blood
Blood Preservation
Clinical trials
Flow Cytometry
FOXP3
Freezing
Granulocytes
Humans
Lymphocytes
Lymphocytes T
lysed frozen whole blood
Population
sample stability
Sodium
Software
Stains & staining
Temperature
Testing laboratories
Treg
United States > US
flow cytometry
lysed frozen whole blood
FOXP3
sample stability
Treg
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Summary:A major challenge for flow cytometry assays supporting clinical trials is postcollection sample stability. Here we present an approach that could mitigate the stability issue while preserving sample integrity and cellular markers, especially when enumerating rare populations such as Tregs. Stability was evaluated using whole blood stored at room temperature and lysed whole blood stored at -80°C. Freezing of lysed whole blood preserved sample integrity and prolonged sample stability for Treg percentage, absolute cell count and median fluorescent intensity values to 11 versus 3 days at room temperature storage. Frozen storage of lysed whole blood can extend sample stability, improve data quality and facilitate sample batch processing during clinical study sample analysis.
ISSN:1757-6180
1757-6199
DOI:10.4155/bio-2020-0080