Lipoxygenase isoenzymes of french bean pericarp: Separation, characterization and chlorophyll-bleaching activity
Two lipoxygenase isoenzymes, Lα and Lβ, were partially purified from pericarp of french bean. The two isoenzymes differed in pI, M r , apparent K M , and lag phase. Both could be categorized as type 2 lipoxygenase, based on their acidic optimum pH, simultaneous production of carbonyl compounds and h...
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Published in | Phytochemistry (Oxford) Vol. 28; no. 4; pp. 1019 - 1024 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Amsterdam
Elsevier Ltd
1989
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | Two lipoxygenase isoenzymes, Lα and Lβ, were partially purified from pericarp of french bean. The two isoenzymes differed in pI,
M
r
, apparent
K
M
, and lag phase. Both could be categorized as type 2 lipoxygenase, based on their acidic optimum pH, simultaneous production of carbonyl compounds and hydroperoxides in the presence of oxygen, aerobic chlorophyll-bleaching activity and inhibition by chlorophyll
a. In the presence of a sufficient amount of preformed hydroperoxides, it was possible to show competitive inhibition of Lα and Lβ by chlorophyll
a. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0031-9422 1873-3700 |
DOI: | 10.1016/0031-9422(89)80175-X |