Preferred co-localization of chromosome 8 and 21 in myeloid bone marrow cells detected by three dimensional molecular cytogenetics
The impact of chromosome architecture in the formation of chromosome aberrations is a recent finding of interphase directed molecular cytogenetic studies. Also positive correlation of translocation frequencies and spatial proximity of chromosomes was described. Thus, disease specific chromosomal tra...
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Published in | International journal of molecular medicine Vol. 24; no. 3; pp. 335 - 341 |
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Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Greece
D.A. Spandidos
01.09.2009
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Subjects | |
Online Access | Get full text |
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Summary: | The impact of chromosome architecture in the formation of chromosome aberrations
is a recent finding of interphase directed molecular cytogenetic studies. Also
positive correlation of translocation frequencies and spatial proximity of chromosomes
was described. Thus, disease specific chromosomal translocations could be due
to tissue specific genomic organization. However, no three-dimensional interphase
fluorescence in situ hybridization (FISH) studies for the nuclear architecture
of bone marrow (BM) cells have previously been done. In this study, BM of three
secondary acute myelogenous leukemia (AML) cases with trisomy 8 and otherwise
normal karyotype were evaluated. Bone marrow cells of one AML and one ALL (acute
lymphoblastic leukemia) case, peripheral blood lymphocytes and human sperm, all
of them with normal karyotype, served as controls. Multicolor banding (MCB) probes
for chromosomes 8 and 21 were applied in suspension-FISH (S-FISH). Interestingly,
in myeloid bone marrow cells chromosomes 8 (di- and trisomic) and 21 tended to
co-localize with their homologue chromosome(s), rather than to be separated. Thus,
the co-localization of chromosomes 8 and 21 might promote a translocation providing
a selective advantage of t(8;21) cells in AML-M2. In summary, the concept that
tissue specific spatial proximity of chromosomes leads to enhanced translocation
frequencies was further supported. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1107-3756 |
DOI: | 10.3892/ijmm_00000237 |