Cortical parvalbumin neurons are responsible for homeostatic sleep rebound through CaMKII activation

• Published in: Nature communications Vol. 15; no. 1; pp. 6054 - 19
• Main Authors: Kon, Kazuhiro, Ode, Koji L., Mano, Tomoyuki, Fujishima, Hiroshi, Takahashi, Riina R., Tone, Daisuke, Shimizu, Chika, Shiono, Shinnosuke, Yada, Saori, Matsuzawa, Kyoko, Yoshida, Shota Y., Yoshida Garçon, Junko, Kaneko, Mari, Shinohara, Yuta, Yamada, Rikuhiro G., Shi, Shoi, Miyamichi, Kazunari, Sumiyama, Kenta, Kiyonari, Hiroshi, Susaki, Etsuo A., Ueda, Hiroki R.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 18.07.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 14/1; 14/63; 38/44; 38/91; 631/337/458/1733; 631/378/1385/1815; 631/378/1385/519; 631/378/2571; 64/110; 64/60; 82/51; 82/58; 82/80; 9/26; Animals; Ca2+/calmodulin-dependent protein kinase II; Calcium ions; Calcium-Calmodulin-Dependent Protein Kinase Type 2 - genetics; Calcium-Calmodulin-Dependent Protein Kinase Type 2 - metabolism; Calmodulin; Cerebral Cortex - metabolism; Excitability; Feedback inhibition; Hibernation; Homeostasis; Humanities and Social Sciences; Kinases; Male; Mice; Mice, Inbred C57BL; Mice, Transgenic; multidisciplinary; Negative feedback; Neurons; Neurons - metabolism; Neurons - physiology; Parvalbumin; Parvalbumins - metabolism; Science; Science (multidisciplinary); Sleep; Sleep - physiology; Sleep and wakefulness; Wakefulness; Wakefulness - physiology
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-024-50168-5

The homeostatic regulation of sleep is characterized by rebound sleep after prolonged wakefulness, but the molecular and cellular mechanisms underlying this regulation are still unknown. In this study, we show that Ca 2+ /calmodulin-dependent protein kinase II (CaMKII)-dependent activity control of parvalbumin (PV)-expressing cortical neurons is involved in homeostatic regulation of sleep in male mice. Prolonged wakefulness enhances cortical PV-neuron activity. Chemogenetic suppression or activation of cortical PV neurons inhibits or induces rebound sleep, implying that rebound sleep is dependent on increased activity of cortical PV neurons. Furthermore, we discovered that CaMKII kinase activity boosts the activity of cortical PV neurons, and that kinase activity is important for homeostatic sleep rebound. Here, we propose that CaMKII-dependent PV-neuron activity represents negative feedback inhibition of cortical neural excitability, which serves as the distributive cortical circuits for sleep homeostatic regulation. The role of cortical PV neurons in the regulation of sleep architecture and homeostatic sleep rebound is not fully understood. Here, the authors show that CaMKII-mediated control of cortical parvalbumin-neuron activity regulates the homeostatic sleep rebound in mice.