Phenolics and antifungal activities analysis in industrial crop Jerusalem artichoke (Helianthus tuberosus L.) leaves

• Published in: Industrial crops and products Vol. 47; pp. 339 - 345
• Main Authors: Chen, Fujia, Long, Xiaohua, Yu, Mengni, Liu, Zhaopu, Liu, Ling, Shao, Hongbo
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.05.2013
• Subjects: Antifungal activity; antifungal agents; antifungal properties; bioassays; Botrytis cinerea; caffeic acid; carbendazim; Colletotrichum gloeosporioides; Dicaffeoylquinic acids; economic valuation; fruits; fungicides; Gibberella zeae; Glomerella cingulata; Helianthus tuberosus; industrial crops; inhibitory concentration 50; Jerusalem artichoke; Jerusalem artichokes; leaf extracts; leaves; minimum inhibitory concentration; Phenolic acids; Phytophthora capsici; Rhizoctonia cerealis; Helianthus tuberosus; Antifungal activity; Jerusalem artichoke; Dicaffeoylquinic acids; Phenolic acids
• ISSN: 0926-6690; 1872-633X
• DOI: 10.1016/j.indcrop.2013.03.027

•Spoilage of industrial crops results in significant losses.•New natural antifungal treatments would have large economic value.•Extracts of antifungal compounds and phenolic acids from Jerusalem artichoke were investigated.•Either crude of the leaf extract or n-butanol fraction was active against Botrytis cinerea and Colletotrichum gloeosporioides. Spoilage of industrial fruits and vegetables in storage and transportation due to fungal infection results in significant losses, and new natural antifungal treatments would have a large economic value. The extracts of antifungal compounds and phenolic acids from Jerusalem artichoke (Helianthus tuberosus L.) leaves were investigated for potential use in enhancing preservation of fruits and vegetables in storage. Either crude leaf extract or n-butanol fraction was active against Botrytis cinerea, Colletotrichum gloeosporioides, Phytophthora capsici Leonian and Rhizoctonia cerealis, with the values of IC50 (half of the maximum inhibitory concentration) ranging from 2.166 to 2.534gL−1 for the crude leaf extract and 0.232–1.911gL−1 for n-butanol fraction. The severity of grey mould caused by B. cinerea was significantly reduced by n-butanol fraction applied at 1 and 2gL−1 (the control efficiency of 71.3% and 77.8%, respectively, compared with commercial preparation Carbendazim. Following in vitro activity-guided fractionation by bioautography, six phenolic acids were separated from n-butanol fraction. Among them, caffeic acid, 3,4-dicaffeoylquinic acid and 1,5-dicaffeoylquinic acid played a dominant role and were active in bioassays against Gibberella zeae, with respective minimum inhibitory concentrations (MIC) being 108, 60 and 4.2μgmL−1. These results imply that Jerusalem artichoke leaves might be a potential source of natural fungicides.