Biomarkers of oxidative stress and protein-protein interaction in chronic obstructive pulmonary disease

• Published in: Archives of physiology and biochemistry Vol. 124; no. 3; pp. 226 - 231
• Main Authors: Aggarwal, Taru, Wadhwa, Ridhima, Rohil, Vishwajeet, Maurya, Pawan Kumar
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 27.05.2018
• Subjects: ascorbic acid; Ascorbic Acid - blood; Case-Control Studies; Chronic obstructive pulmonary disease; Female; Gene Expression Regulation, Enzymologic; Humans; Male; Malondialdehyde - blood; Matrix Metalloproteinase 12 - blood; Middle Aged; MMP-12; MDA; Oxidative Stress; PPI network; Protein Interaction Maps; Pulmonary Disease, Chronic Obstructive - blood; Pulmonary Disease, Chronic Obstructive - metabolism; uric acid; Uric Acid - blood; PPI network; ascorbic acid; Chronic obstructive pulmonary disease; MMP-12; MDA; uric acid; oxidative stress
• ISSN: 1381-3455; 1744-4160; 1744-4160
• DOI: 10.1080/13813455.2017.1387796

Content: The increased oxidative stress in chronic obstructive pulmonary disease (COPD) patients is the result of increased inhaled oxidants, generated by various cells of the airways. Objective: The investigation included measurements of malondiadehyde (MDA), uric acid, ascorbic acid, and matrix metalloproteinase-12 (MMP-12) in COPD patient. We also performed genetic analysis for protein-protein interaction (PPI) network. Materials and methods: The study was conducted on healthy subjects with normal lung function (NS, 14 subjects) and 28 patients (Global Initiative for Chronic Obstructive Lung Disease (Gold) 1 and Gold 2) with COPD. Results: There was significant (p < .001) increase in MMP-12, MDA and uric acid levels as compared to healthy controls. A significant (p < .001) decline in ascorbic acid level was observed in COPD patients. The PPI was found to be 0.833 which indicated that proteins present in COPD are linked. Discussion and conclusion: This study suggests oxidative stress plays an important role in COPD and the PPI provide indication that proteins present in COPD are linked.