Metribuzin resistance in photoautotrophic Chenopodium rubrum cell cultures : Characterization of double and triple mutations in the psbA gene

• Published in: FEBS letters Vol. 329; no. 1; pp. 43 - 46
• Main Authors: Schwenger-Erger, Claudia, Thiemann, Jutta, Barz, Wolfgang, Johanningmeier, Udo, Naber, Dirk
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 23.08.1993; Elsevier
• Subjects: Amino Acid Sequence; Bacterial Proteins - genetics; Base Sequence; Biological and medical sciences; Cells, Cultured; Chenopodium rubrum; Classical genetics, quantitative genetics, hybrids; Cloning, Molecular; D1 protein; DNA - chemistry; DNA - genetics; Drug Resistance - genetics; Fundamental and applied biological sciences. Psychology; Genes, Plant; Genetics of eukaryotes. Biological and molecular evolution; Herbicide; Herbicide resistance; Herbicides - pharmacology; Molecular Sequence Data; Mutation; Photosynthetic Reaction Center Complex Proteins - genetics; Photosystem II; Photosystem II Protein Complex; Plant Proteins - genetics; Plants - drug effects; Plants - genetics; Polymerase Chain Reaction; psbA gene; Pteridophyta, spermatophyta; Triazines - pharmacology; Vegetals; DCMU, 3-(3,4-dichlorophenyl)-1,1-dimethylurea; Herbicide resistance; D1 protein; kDa, kilodalton; Photosystem II; psbA gene; bromacil, 5-bromo-3-(sec-butyl)-6-methyl-uracil; metribuzin, 4-amino-6-(tertbutyl)-3-methylthio-as-triazine-5(4H)-one; Herbicide; dinoseb, 2,4-dinitro-6-sec-butylphenol; RFLP, restriction fragment length polymorphism; propanil, N-(3,4-dichlorophenyl)propionanüde; phenmedipham, methyl m-hydroxy-carbanilate m-methylcarbanilate; Q A and Q B, primary and secondary quinone acceptors; atrazine, 2-chloro-4-(ethylamino)-6-(iso-propyl-amino)- s-triazine; PCR, polymerase chain reaction; Chenopodium rubrum; PSII, photosystem II; Resistance; Site specificity; Cell culture; Dicotyledones; Angiospermae; Metribuzin; Spermatophyta; Mutation; Chenopodiaceae
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(93)80189-2

Sequence analyses of eight metribuzin-resistant mutants of photoautotrophic Chenopodium rubrum cell cultures revealed new mutations in the psbA gene coding for the 32 kDa herbicide binding protein. Mutants were found to possess either two or three changes in the amino acid sequence of the D1-protein between positions 219 and 272.