One-pot preparation of nano-scaled magnetic-pectin particles (Fe3O4@pectin NPs): cytotoxicity, antioxidant, and anti-liver cancer properties

• Published in: Journal of experimental nanoscience Vol. 17; no. 1; pp. 326 - 338
• Main Authors: Zhang, Qiao, Cui, Wenyu, Guo, Honglin, Wang, Baoqing, Wang, He, Zhang, Jimei, Li, Wenlan
• Format: Journal Article
• Language: English
• Published: Abingdon Taylor & Francis 31.12.2022; Taylor & Francis Ltd; Taylor & Francis Group
• Subjects: and Anti-liver cancer; Antioxidant; Antioxidants; Biological effects; Chemical precipitation; Cytotoxicity; Infrared spectroscopy; Iron oxides; Liver cancer; Magnetic; Magnetic properties; Magnetometers; Nanocomposite; Nanoparticles; Pectin; Spectrum analysis; Synthesis; Toxicity
• ISSN: 1745-8080; 1745-8099
• DOI: 10.1080/17458080.2022.2063279

This work described the one-pot synthesis of orange pectin encapsulated Fe3O4 nanoparticles (Fe 3 O 4 @Pectin NPs) which is prepared by co-precipitation of Fe(II/(III) ions in alkaline solution mediated by pectin. This process led to formation of magnetic nanoparticles within the network of pectin. Physicochemical characterization of the as-synthesized Fe 3 O 4 @Pectin NPs was carried out through Fourier transformed infrared spectroscopy (FT-IR), electron microscopy (SEM and TEM), energy dispersive X-ray spectroscopy (EDX), vibrating sample magnetometer (VSM) and X-ray diffraction (XRD). The in vitro cytotoxic and anti-liver cancer effects of biologically synthesized Fe 3 O 4 @Pectin NPs against pleomorphic hepatocellular carcinoma (SNU-387), hepatic ductal carcinoma (LMH/2A), morris hepatoma (McA-RH7777), and novikoff hepatoma (N1-S1 Fudr) cancer cell lines were assessed. The anti-liver cancer properties of the Fe 3 O 4 @Pectin NPs could significantly remove pleomorphic hepatocellular carcinoma (SNU-387), hepatic ductal carcinoma (LMH/2A), morris hepatoma (McA-RH7777), and novikoff hepatoma (N1-S1 Fudr) cancer cell lines in a time and concentration-dependent manner by MTT assay. The IC 50 of the Fe 3 O 4 @Pectin NPs were 8, 13, 10, and 7 µg/mL against pleomorphic hepatocellular carcinoma (SNU-387), hepatic ductal carcinoma (LMH/2A), morris hepatoma (McA-RH7777), and novikoff hepatoma (N1-S1 Fudr) cancer cell lines. The antioxidant activity of Fe 3 O 4 @Pectin NPs was determined by DPPH method. The Fe 3 O 4 @Pectin NPs showed the high antioxidant activity according to the IC 50 value. It seems that the anti-human liver cancer effect of recent nanoparticles is due to their antioxidant effects.