The effects of Zataria multiflora aromatic water and nano emulsion of Z. multiflora essential oil on the serum level of IFN-γ and IL-4 in mice with hydatidosis

• Published in: Journal of parasitic diseases Vol. 45; no. 3; pp. 733 - 737
• Main Authors: Parhizgar, N., Borji, H., Moazeni, M., Saboor Darbandi, M., Heidarpour, M.
• Format: Journal Article
• Language: English
• Published: New Delhi Springer India 01.09.2021; Springer Nature B.V
• Subjects: Albendazole; Cytokines; Drinking water; Essential oils; Health Promotion and Disease Prevention; Hydatid disease; Infectious Diseases; Interleukin 4; Lymphocytes T; Medicine; Medicine & Public Health; Oils & fats; Original; Original Article; Zataria multiflora; γ-Interferon; IL-4; IFN-γ; Nano emulsion; Hydatidosis
• ISSN: 0971-7196; 0975-0703
• DOI: 10.1007/s12639-021-01353-6

The aim of the present investigation was to evaluate the impacts of Zataria multiflora aromatic water (ZMAW) and nano emulsion (NE) of Z. multiflora essential oil (ZMEO) on IL-4 (Th2-derived cytokine) and IFN-γ (Th1-derived cytokine) concentrations in mice with hydatidosis. Sixty four healthy BALB/c female mice were intraperitoneally injected by 1500 live protoscoleces of Echinococcus granulosus . Five month after injection, the infected mice were divided into seven treatment groups, including: Albendazole (100 mg/kg), ZMAW (100 ml/L), Albendazole 100 + ZMAW (Albendazole 100 mg/kg and ZMAW 100 ml/L), Albendazole 50 + ZMAW (Albendazole 50 mg/kg and ZMAW 100 ml/L), Albendazole 100 + NE of ZMEO (Albendazole 100 mg/kg and NE of ZMEO 10 ml/L), albendazole 50 + NE of ZMEO (albendazole 50 mg/kg and NE of ZMEO 10 ml/L) and the control group with no treatment. The mice received the ZMAW, NE of ZMEO and/or albendazole through drinking water for 90 days. At the end of treatment (90 days), blood specimens were harvested from all mice. A significant reduction in IL-4 concentration was revealed in the albendazole 50 group + NE of ZMEO, when compared to the control group ( P  < 0.05). No significant difference was noticed for the IFN-γ concentration between the trial groups. Increased IFN-γ: IL-4 ratio was observed in the albendazole 50 + ZMAW, Albendazole 50 + NE of ZMEO, Albendazole 100 + NE of ZMEO groups in comparison with the control group ( P  < 0.05). The findings observed in the current study showed that the administration of ZMAW and NE of ZMEO along with albendazole could enhance the IFN-γ: IL-4 ratio (Th1/Th2 balance), which may have therapeutic value in the treatment of hydatid disease.