Detection of D2-40 monoclonal antibody-labeled lymphatic vessel invasion in esophageal squamous cell carcinoma and its clinicopathologic significance

• Published in: Cancer biology & medicine Vol. 10; no. 2; pp. 81 - 85
• Main Authors: Bai, Bing, Ma, Wei, Wang, Kai, Ha, Sita, Wang, Jian-Bo, Tan, Bing-Xu, Wang, Na-Na, Yang, Sheng-Si, Jia, Yi-Bin, Cheng, Yu-Feng
• Format: Journal Article
• Language: English
• Published: China 2Department of Oncology, Yiyuan Chinese edicine Hospital, Zibo 256100, China%Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan 250012, China%Department of Oncology, Wendeng Central Hospital, Weihai 264400, China%Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto 8600862, Japan 01.06.2013; Department of Radiation Oncology, Qilu Hospital of Shandong University, Jinan 250012, China; Chinese Anti-Cancer Association; China Anti-Cancer Association
• Subjects: D2-40; Esophageal squamous cell carcinoma; lymph node metastasis; lymphatic vessel invasion; Original; prognosis; 入侵; 单克隆抗体; 多元回归分析; 标记; 检测; 淋巴管; 食管癌; 鳞状细胞癌; Esophageal squamous cell carcinoma; prognosis; D2-40; lymph node metastasis; lymphatic vessel invasion
• ISSN: 2095-3941; 2095-3941
• DOI: 10.7497/j.issn.2095-3941.2013.02.003

Objective： This study aims to investigate the clinicopathologic significance of lymphatic vessel invasion （LVI） labeled by D2-40 monoclonal antibody in esophageal squamous cell carcinoma （ESCC）. Methods： Immunohistochemical assay was used to detect the expression of D2-40 and LVI in 107 ESCC patients. Then, the correlation between the clinicopathologic feature and the overall survival time of the patients was analyzed. Results： The lymph node metastasis rates were 70% and 21% in the LVI-positive and LVI-negative groups, respectively. The nodal metastasis rate was higher in the LVI-positive group than in the LVI-negative group. Multivariate regression analysis showed that LVI was related to nodal metastasis （P〈0.001）. The median survival time of the patients was 26 and 43 months in the LVI-positive and LVI-negative groups, respectively. Mthough univariate regression analysis showed significant difference between the two groups （P=0.014）, multivariate regression analysis revealed that LVI was not an independent prognostic factor for overall survival in the ESCC patients （P=0.062）. Lymphatic node metastasis （P=0.031）, clinical stage （P=0.019）, and residual tumor （P=0.026） were the independent prognostic factors. Conclusion： LVI labeled by D2-40 monoclonal antibody is a risk factor predictive of lymph node metastasis in ESCC patients.