Isolation and Characterization of Two Glycoproteins from Patients with Alveolar Proteinosis

The saline-insoluble particulate material obtained by pulmonary lavage from four patients with alveolar proteinosis was analyzed for total lipid (52%), protein (44%), and carbohydrate (4%). Sodium dodecyl sulfate-gel electrophoresis revealed only three major peptides in these patients. Molecular wei...

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Published inProceedings of the National Academy of Sciences - PNAS Vol. 70; no. 4; pp. 973 - 976
Main Authors Passero, Michael A., Tye, Ross W., Kilburn, Kaye H., Lynn, William S.
Format Journal Article
LanguageEnglish
Published United States National Academy of Sciences of the United States of America 01.04.1973
National Acad Sciences
Subjects
Amino Acids - analysis
Antigen-Antibody Reactions
Biological Sciences: Biochemistry
Carbohydrates - analysis
Cell membranes
Chromatography, Gel
Electrophoresis
Epitopes
Gels
Glycoproteins
Glycoproteins - analysis
Glycoproteins - isolation & purification
Humans
Lipids
Lungs
Materials
Molecular weight
Peptides - analysis
Pulmonary Alveolar Proteinosis - metabolism
Pulmonary alveoli
Therapeutic irrigation
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Summary:The saline-insoluble particulate material obtained by pulmonary lavage from four patients with alveolar proteinosis was analyzed for total lipid (52%), protein (44%), and carbohydrate (4%). Sodium dodecyl sulfate-gel electrophoresis revealed only three major peptides in these patients. Molecular weights (from the gels) were 69,000, 62,000, and 36,000, and the latter two peptides were periodic acid-Schiff stain-positive. Amino-acid and sugar analyses were performed on the peptides cut from the gels and on peptides purified by Sephadex G-200 chromatography. The 69,000-molecular-weight peptide contained no carbohydrate. Antibody studies and amino-acid analysis indicated that it was albumin. The 62,000-molecular-weight peptide contained 1% hydroxyproline, 1% hydroxylysine, 10% glycine, and 9% carbohydrate. The 36,000-molecular-weight peptide contained 1.2% hydroxyproline, 1% hydroxylysine, 13% glycine, 1.4% sialic acid, 2.6% glucose, 2.4% galactose, 2% mannose, 0.8% fucose, 0.4% glycosamine, and 0.6% galactosamine. Proteins extracted from kidney glomeruli with similar amino-acid and carbohydrate composition have been observed previously.
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Address all correspondence and reprint requests to Dr. Michael A. Passero, Box M3804, Duke University Medical Center, Durham, N.C. 27710.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.70.4.973