Quantitative analysis of Gd@C82(OH)22 and cisplatin uptake in single cells by inductively coupled plasma mass spectrometry

Cisplatin is a commonly used chemotherapeutic drug in cancer treatment, whereas Gd@C 82 (OH) 22 is a new nanomaterial anti-tumor agent. In this study, we determined intracellular Gd@C 82 (OH) 22 and cisplatin after treatment of Hela and 16HBE cells by single cell inductively coupled plasma-mass spec...

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Published inAnalytical and bioanalytical chemistry Vol. 407; no. 9; pp. 2383 - 2391
Main Authors Zheng, Ling-Na, Wang, Meng, Zhao, Lei-Chao, Sun, Bao-Yun, Wang, Bing, Chen, Han-Qing, Zhao, Yu-Liang, Chai, Zhi-Fang, Feng, Wei-Yue
Format Journal Article
LanguageEnglish
Published Berlin/Heidelberg Springer Berlin Heidelberg 01.03.2015
Subjects
Analytical Chemistry
Antineoplastic Agents - analysis
Antineoplastic Agents - metabolism
Biochemistry
Cell Line, Tumor
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Cisplatin - analysis
Cisplatin - metabolism
Food Science
Gadolinium - analysis
Gadolinium - metabolism
Humans
Laboratory Medicine
Mass Spectrometry - methods
Monitoring/Environmental Analysis
Nanostructures - chemistry
Neoplasms - chemistry
Neoplasms - metabolism
Research Paper
Single-Cell Analysis - methods
Spectrochemical Plasmas for Clinical and Biochemical Analysis
Single cell
Gd@C
OH
ICP-MS
Cisplatin
Quantitative analysis
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Summary:Cisplatin is a commonly used chemotherapeutic drug in cancer treatment, whereas Gd@C 82 (OH) 22 is a new nanomaterial anti-tumor agent. In this study, we determined intracellular Gd@C 82 (OH) 22 and cisplatin after treatment of Hela and 16HBE cells by single cell inductively coupled plasma-mass spectrometry (SC-ICP-MS), which could provide quantitative information at a single-cell level. The cell digestion method validated the accuracy of the SC-ICP-MS. The concentrations of Gd@C 82 (OH) 22 and cisplatin in cells at different exposure times and doses were studied. The SC-ICP-MS is a promising complement to available methods for single cell analysis and is anticipated to be applied further to biomedical research. Graphical Abstract The quantitative results of Gd@C 82 (OH) 22 in single cells determined by SC-ICP-MS and acid digestion method, respectively
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ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-014-8422-3