Primary structure of the histone H3 and H4 genes and their flanking sequences in a minor histone gene cluster of Xenopus laevis

• Published in: FEBS letters Vol. 136; no. 1; pp. 45 - 52
• Main Authors: Moorman, A.F.M., De Boer, P.A.J., De Laaf, R.T.M., Van Dongen, W.M.A.M., Destrée, O.H.J.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 21.12.1981
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Biological Evolution; Codon; DNA; DNA Restriction Enzymes; Histones - genetics; Nucleic Acid Hybridization; Oogenesis; Species Specificity; Xenopus laevis - embryology
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(81)81211-2

Here, the authors report the nucleotide sequences of genes coding for histones H3 and H4 and their surrounding regions containing putative regulatory sequences. The H3 coding sequence shows 18% base substitutions, resulting in 6 amino acid changes, compared with a gene for histone H3 from the sea urchin P. miliaris (clone h19). The H4 gene has 21% nucleotide divergence resulting in only one amino acid substitution compared with the H4 gene in h19. The 3'-flanking regions of both the H3 and H4 genes display a striking homology with those of P. miliaris histone genes in h19, especially with regard to the palindromic structure GGCTCTTTTCAGAGCC. The 5'-flanking regions share with other histone genes several regions of homology