Salt-induced hypertension in Dahl salt-resistant and salt-sensitive rats with NOS II inhibition
Whitaker Cardiovascular Institute, Evans Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118-2394 Although recent evidence suggests that reduced nitric oxide (NO) production may be involved in salt-induced hypertension, the specific NO synthase (NOS) responsible...
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Published in | American journal of physiology. Heart and circulatory physiology Vol. 277; no. 2; pp. H732 - H739 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
01.08.1999
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Subjects | |
Online Access | Get full text |
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Summary: | Whitaker Cardiovascular Institute, Evans Department of Medicine,
Boston University School of Medicine, Boston, Massachusetts 02118-2394
Although recent
evidence suggests that reduced nitric oxide (NO) production may be
involved in salt-induced hypertension, the specific NO synthase (NOS)
responsible for the conveyance of salt sensitivity remains unknown. To
determine the role of inducible NOS (NOS II) in salt-induced
hypertension, we treated Dahl salt-resistant (DR) rats with the
selective NOS II inhibitor 2-amino-5,6-dihydro-6-methyl-4H-1,3-thiazine
(AMT) for 12 days. Tail-cuff systolic blood pressures rose 29 ± 6 and 42 ± 8 mmHg in DR rats given 150 and 300 nmol AMT/h,
respectively ( P < 0.01, 2-way ANOVA)
after 7 days of 8% NaCl diet. We observed similar results
with two other potent selective NOS II inhibitors,
S -ethylisourea (EIT) and
N -[3-(aminomethyl)benzyl]acetamidine
hydrochloride (1400W). Additionally, AMT effects were independent of
alterations in endothelial function as assessed by diameter change of
mesenteric arterioles in response to methacholine using
videomicroscopy. We, therefore, conclude from these data that NOS II is
important in salt-induced hypertension.
salt-sensitive hypertension; Dahl rats; N G -nitro- L -arginine methyl ester; 2-amino-5,6-dihydro-6-methyl-4H-1,3-thiazine; N -[3-(aminomethyl)benzyl]acetamidine
hydrochloride |
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ISSN: | 0363-6135 0002-9513 1522-1539 2163-5773 |
DOI: | 10.1152/ajpheart.1999.277.2.h732 |