Monoamine receptors in human corneal epithelium and endothelium

• Published in: Acta ophthalmologica Scandinavica Vol. 84; no. 1; pp. 110 - 115
• Main Authors: Grueb, Matthias, Wallenfels‐Thilo, Barbara, Denk, Oliver, Mielke, Joerg, Reinthal, Eva, Rohrbach, Jens Martin, Bartz‐Schmidt, Karl Ulrich
• Format: Journal Article
• Language: English
• Published: Oxford, UK; Malden, USA Blackwell Publishing Ltd/Inc 01.02.2006
• Subjects: Adult; Aged; Blotting, Western; cornea; endothelium; Endothelium, Corneal - metabolism; epithelium; Epithelium, Corneal - metabolism; Female; Fluorescent Antibody Technique, Indirect; Humans; Immunoblotting; Immunohistochemistry; Male; Middle Aged; monoamine‐receptor; Receptors, Adrenergic - metabolism; Receptors, Dopamine - metabolism; Receptors, Serotonin - metabolism
• ISSN: 1395-3907; 1600-0420
• DOI: 10.1111/j.1600-0420.2005.00551.x

. Purpose:  Monoamine receptors are found throughout the body. Reports about the presence of monoamine receptors in the human cornea are inconsistent. Methods:  Immunohistochemistry, immunofluorescence and immunoblotting were used to localize monoamine receptor sites on human corneal epithelium and endothelium. Results:  Antibodies to alpha‐1, beta‐1 and beta‐2 adrenergic receptors and to D1‐like and 5HT‐7 receptors were bound in corneal epithelium. Antibodies to alpha‐1, alpha‐2A, beta‐1 and beta‐2 adrenergic receptors and to 5HT‐7 receptors were bound in corneal endothelium. Conclusions:  Our data demonstrate the presence of several monoamine receptors in the human cornea. These receptors may play a role in the regulation of fluid transport or corneal homeostasis.