Role of parietal and principal gastric mucosa cells in the phenomenon of concentration of aluminum and indium

• Published in: Microscopy research and technique Vol. 75; no. 2; pp. 182 - 188
• Main Authors: Maghraoui, Samira, Ayadi, Ahlem, Audinot, Jean-Nicolas, Ammar, Aouatef Ben, Jaafoura, Mohamed-Habib, Hili, Ali El, Migeon, Henri-Noël, Tekaya, Leila
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.02.2012
• Subjects: Aluminum Compounds - administration & dosage; Aluminum Compounds - pharmacokinetics; Animals; Electron Probe Microanalysis; gastric cells; Gastric Mucosa - anatomy & histology; Gastric Mucosa - drug effects; Gastric Mucosa - metabolism; III-A group elements; Indium - administration & dosage; Indium - pharmacokinetics; Liver - drug effects; Liver - metabolism; Liver - ultrastructure; Lysosomes - drug effects; Lysosomes - ultrastructure; Male; Microscopy, Electron, Transmission; Nitrates - administration & dosage; Nitrates - pharmacokinetics; Parietal Cells, Gastric - drug effects; Parietal Cells, Gastric - metabolism; Parietal Cells, Gastric - ultrastructure; Rats; Rats, Wistar; secondary ion mass spectrometry; Spectrometry, Mass, Secondary Ion; transmission electron microscopy
• ISSN: 1059-910X; 1097-0029
• DOI: 10.1002/jemt.21041

The subcellular behavior of aluminum and indium, used in medical and industrial fields, was studied in the gastric mucosa and the liver after their intragastric administration to rats, using, two of the most sensitive methods of observation and microanalysis, the transmission electron microscopy, and the secondary ion mass spectrometry. The ultrastructural study showed the presence of electron dense deposits, in the lysosomes of parietal and principal gastric mucosa cells but no loaded lysosomes were observed in the different studied hepatic territories. The microanalytical study allowed the identification of the chemical species present in those deposits as aluminum or indium isotopes and the cartography of their distribution. No modification was observed in control rats tissues. In comparison to previous studies describing the mechanism of aluminum concentration in the gastric mucosa and showing that this element was concentrated in the lysosomes of fundic and antral human gastric mucosa, our study provided additional informations about the types of cells involved in the phenomenon of concentration of aluminum and indium, which are the parietal and the principal cells of the gastric mucosa. Our study demonstrated that these cells have the ability to concentrate selectively aluminum and indium in their lysosomes, as a defensive reaction against intoxication by foreign elements. Microsc. Res. Tech., 2011 © 2011 Wiley Periodicals, Inc.