Isolation and characterization of a very virulent Infectious bursal disease virus from turkey

Infectious bursal disease (IBD) is a highly contagious disease of chickens caused by Infectious bursal disease virus (IBDV). In turkeys, however, infection with classical virulent IBDV strains lead only to subclinical forms of the disease. We attempted to isolate IBDV from the bursa of turkey and ch...

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Bibliographic Details
Published inActa virologica (Anglickâa verze) Vol. 53; no. 4; p. 271
Main Authors Razmyar, J, Peighambari, S M
Format Journal Article
LanguageEnglish
Published Slovakia 01.01.2009
Subjects
Animals
Birnaviridae Infections - epidemiology
Birnaviridae Infections - veterinary
Birnaviridae Infections - virology
Infectious bursal disease virus - classification
Infectious bursal disease virus - genetics
Infectious bursal disease virus - isolation & purification
Infectious bursal disease virus - pathogenicity
Iran - epidemiology
Phylogeny
Poultry Diseases - epidemiology
Poultry Diseases - virology
Restriction Mapping
Sequence Analysis, DNA
Turkeys - virology
Viral Structural Proteins - genetics
Viral Structural Proteins - metabolism
Virulence
Iran
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Summary:Infectious bursal disease (IBD) is a highly contagious disease of chickens caused by Infectious bursal disease virus (IBDV). In turkeys, however, infection with classical virulent IBDV strains lead only to subclinical forms of the disease. We attempted to isolate IBDV from the bursa of turkey and characterize it. Amplification of a 743-bp fragment of VP2 gene by RT-PCR and restriction analysis of the product showed a pattern compatible with very virulent IBDV (vvIBDV). Comparison of the sequence of this isolate with those of other IBDVs and phylogenetic analysis confirmed very virulent nature of the isolate. This is the first report on the isolation of vvIBDV from turkey in Iran.
ISSN:0001-723X
DOI:10.4149/av_2009_04_271