Synthesis of bioactive silver nanoparticles using alginate, fucoidan and laminaran from brown algae as a reducing and stabilizing agent

• Published in: Carbohydrate polymers Vol. 245; p. 116547
• Main Authors: Yugay, Y.A., Usoltseva, R.V., Silant'ev, V.E., Egorova, A.E., Karabtsov, A.A., Kumeiko, V.V., Ermakova, S.P., Bulgakov, V.P., Shkryl, Y.N.
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.10.2020
• Subjects: Agrobacterium tumefaciens - drug effects; Alginates - chemistry; Alginates - isolation & purification; Animals; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Catalysis; Cell Line, Tumor; Cell Proliferation - drug effects; Cell Survival - drug effects; Cytotoxicity; Escherichia coli - drug effects; Excipients - chemistry; Fucus evanescens; Glucans - chemistry; Glucans - isolation & purification; Green synthesis; Metal Nanoparticles - chemistry; Nanosilver; Phaeophyceae - chemistry; Polysaccharides - chemistry; Polysaccharides - isolation & purification; Rats; Reducing Agents - chemistry; Saccharina cichorioides; Seaweed - chemistry; Silver - chemistry; Saccharina cichorioides; Cytotoxicity; Antibacterial activity; Green synthesis; Nanosilver; Fucus evanescens
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2020.116547

•The highly purified fractions of algal laminaran, fucoidan and alginate were isolated.•Seaweed polysaccharides catalyzed the formation of silver nanoparticles.•Alginate possessed the highest reduction potential among studied polysaccharides.•The cytotoxicity of nanoparticles varied depending on the properties of polysaccharides.•Silver nanoparticles had antibacterial activity against plant and animal pathogens. In this report, polysaccharides – alginate, fucoidan, laminaran – were isolated from marine algae Saccharina cichorioides and Fucus evanescens and their activity as a reducing and stabilizing agents in the biogenic synthesis of silver nanoparticles was evaluated. The cytotoxic and antibacterial properties of obtained nanoparticles were also assessed. It was found that all tested polysaccharides could be used as a reducing agent; however, their catalytic activities varied significantly in the following range alginate < fucoidan < laminaran. Nanoparticles demonstrated cytotoxicity against rat C6 glioma cells. It was considerably higher for alginate- and laminaran-obtained nanosilver samples compared to fucoidan. Additionally, silver nanoparticles possessed considerable antibacterial properties more pronounced in fucoidan-obtained samples. Our data demonstrate that different algal polysaccharides can be used for the synthesis of silver nanoparticles with varying bioactivities.