EGCG inhibited bladder cancer SW780 cell proliferation and migration both in vitro and in vivo via down-regulation of NF-κB and MMP-9

• Published in: The Journal of nutritional biochemistry Vol. 41; pp. 56 - 64
• Main Authors: Luo, Ke-Wang, Wei Chen, Lung, Wing-Yin, Wei, Xia-Yun, Cheng, Bao-Hui, Cai, Zhi-Ming, Huang, Wei-Ren
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.03.2017
• Subjects: Animals; Antineoplastic Agents, Phytogenic - administration & dosage; Antineoplastic Agents, Phytogenic - adverse effects; Antineoplastic Agents, Phytogenic - metabolism; Antineoplastic Agents, Phytogenic - therapeutic use; Apoptosis - drug effects; Bladder cancer; Catechin - administration & dosage; Catechin - adverse effects; Catechin - analogs & derivatives; Catechin - metabolism; Catechin - therapeutic use; Cell Line, Tumor; Dose-Response Relationship, Drug; Down-Regulation - drug effects; EGCG; Female; Humans; Injections, Intraperitoneal; Matrix Metalloproteinase 9 - chemistry; Matrix Metalloproteinase 9 - genetics; Matrix Metalloproteinase 9 - metabolism; Mice, Inbred BALB C; Mice, Nude; MMP-9; Neoplasm Invasiveness; Neoplasm Proteins - antagonists & inhibitors; Neoplasm Proteins - genetics; Neoplasm Proteins - metabolism; NF-kappa B - antagonists & inhibitors; NF-kappa B - metabolism; NF-κB; Random Allocation; Specific Pathogen-Free Organisms; SW780; Tumor Burden - drug effects; Urinary Bladder - drug effects; Urinary Bladder - metabolism; Urinary Bladder - pathology; Urinary Bladder Neoplasms - drug therapy; Urinary Bladder Neoplasms - metabolism; Urinary Bladder Neoplasms - pathology; Xenograft Model Antitumor Assays; SW780; NF-κB; Bladder cancer; MMP-9; EGCG
• ISSN: 0955-2863; 1873-4847
• DOI: 10.1016/j.jnutbio.2016.12.004

Epigallocatechin-3-gallate (EGCG), the bioactive polyphenol in green tea, has been demonstrated to have various biological activities. Our study aims to investigate the antiproliferation and antimigration effects of EGCG against bladder cancer SW780 cells both in vitro and in vivo. Our results showed that treatment of EGCG resulted in significant inhibition of cell proliferation by induction of apoptosis, without obvious toxicity to normal bladder epithelium SV-HUC-1 cells. EGCG also inhibited SW780 cell migration and invasion at 25–100 μM. Western blot confirmed that EGCG induced apoptosis in SW780 cells by activation of caspases-8, -9 and -3, Bax, Bcl-2 and PARP. Besides, animal study demonstrated that EGCG [100 mg/kg, intraperitoneal (i.p.) injection daily for 3 weeks] decreased the tumor volume significantly in mice bearing SW780 tumors, as well as the tumor weight (decreased by 68.4%). In addition, EGCG down-regulated the expression of nuclear factor-kappa B (NF-κB) and matrix metalloproteinase (MMP)-9 in both protein and mRNA level in tumor and SW780 cells. When NF-κB was inhibited, EGCG showed no obvious effect in cell proliferation and migration. In conclusion, our study demonstrated that EGCG was effective in inhibition SW780 cell proliferation and migration, and presented first evidence that EGCG inhibited SW780 tumor growth by down-regulation of NF-κB and MMP-9.