In-vitro assessment of a novel plant rhizobacterium, Citrobacter freundii, for degrading and biocontrol of food mycotoxin deoxynivalenol

• Published in: Toxicon (Oxford) Vol. 227; p. 107095
• Main Authors: Murtaza, Bilal, Li, Xiaoyu, Dong, Liming, Saleemi, Muhammad Kashif, Iqbal, Mazhar, Majeed, Saima, Ali, Ashiq, Li, Gen, Jin, Bowen, Wang, Lili, Xu, Yongping
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.05.2023
• Subjects: Animals; Bacteria; Bacteria - metabolism; Biodegradation; Chromatography, Liquid; Citrobacter freundii; Citrobacter freundii - metabolism; Deoxynivalenol (DON); Food Contamination - analysis; Food Contamination - prevention & control; High performance liquid chromatography (HPLC); Mycotoxins - analysis; Tandem Mass Spectrometry; Biodegradation; Deoxynivalenol (DON); Bacteria; High performance liquid chromatography (HPLC); Citrobacter freundii
• ISSN: 0041-0101; 1879-3150
• DOI: 10.1016/j.toxicon.2023.107095

Deoxynivalenol (DON) is one of the most harmful and well-known toxins present in food and animal feed throughout the world. Citrobacter freundii (C. freundii-ON077584), a novel DON-degrading strain, was isolated from rice root-linked soil samples. The degrading properties, including DON concentrations, incubation pH, incubation temperatures, bacterial concentrations, and acid treatment effect on degradation, were evaluated. At pH 7 and an incubation temperature of 37 °C, C. freundii demonstrated the capability to degrade more than 90% of DON. The degraded products of DON were identified as 3-keto-DON and DOM-1, which were confirmed by High Performance Liquid Chromatography (HPLC) and Ultra-Performance Liquid Chromatography hyphenated with Tandem Mass Spectrometry (UPLC-MS/MS) analyses. The mechanism of DON degradation into 3-keto-DON and DOM-1 by this bacterial strain will be further explored to identify and purify novel degrading enzymes that can be cloned to the microorganism and added to the animal feed to degrade the DON in the digestion tract. [Display omitted] •Plant rhizobacterial strain against deoxynivalenol was isolated and identified as Citrobacter freundii.•Citrobacter freundii was able to degrade deoxynivalenol more than 90% in Luria-Bertani medium.•The deoxynivalenol degrading products were 3-keto-DON and DOM-1 as confirmed by Ultra-Performance Liquid Chromatography.•Degrading properties of rhizobacterial strain were also examined.