Hepatoprotective efficacy of Premna integrifolia L. leaves against aflatoxin B1-induced toxicity in mice

• Published in: Toxicon (Oxford) Vol. 166; pp. 88 - 100
• Main Authors: Singh, Chandrashekhar, Prakash, Chandra, Mishra, Pallavi, Tiwari, Kavindra Nath, Mishra, Sunil Kumar, More, Raghunath Shahaji, Kumar, Vijay, Singh, Jasmeet
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01.08.2019
• Subjects: Aflatoxin B1; Apoptosis; Hepatotoxicity; Oxidative stress; Premna integrifolia; Oxidative stress; PCV; AST; ALP; UPLC-QTOF-MS/MS; ALT; SOD; AFB1; MDA; RBC; ROS; Aflatoxin B1; WBC; Hb; Premna integrifolia; EEPL; PL; Hepatotoxicity; GSH; Apoptosis
• ISSN: 0041-0101; 1879-3150
• DOI: 10.1016/j.toxicon.2019.05.014

The present study evaluated the hepatoprotective role of ethanol extract of P. integrifolia leaves (EEPL) on aflatoxin B1 (AFB1)-induced toxicity in mice. Mice were administered with AFB1 (0.1 mg/kg b. wt., orally) for 90 days, EEPL (400 and 600 mg/kg b. wt., orally) and silymarin (100 mg/kg b. wt., orally) in combination with AFB1. The study shows the protective effect of EEPL by the restoration of altered hematological indices and liver marker enzymes. Restoration of lipid peroxidation and glutathione content, along with activities of antioxidant enzymes, suggest amelioration of oxidative stress in AFB1-intoxicated mice. In addition, EEPL attenuated apoptosis and histopathological alterations in liver tissue. In conclusion, the current study suggests that EEPL protect mice liver against AFB1 toxicity by inhibiting oxidative stress and apoptosis. The protective activity of EEPL may be due to the enrichment of flavonoids (neohesperidin, apigenin-7-O-glucoside, catechin hydrate, cyanidin chloride, quercetin-3-galactoside, diosmin, genistein, malvin chloride, 4-hydroxy-3-methoxycinnamic acid, kaempferol-3-O-alpha-L-arabinoside, myricitrin, poncirin, vitexin and tiliroside) in the extract as identified by UPLC-QTOF-MS/MS. •Hepatotoxicity was induced byAFB1 in mice by oral administration.•Administration of EEPL restored the hematological and serum indices.•EEPL decreased oxidative stress and liver damage in AFB1-intoxicated mice.•EEPL treatment attenuated apoptosis (Caspase-3, Bax Bcl-2 mRNA levels).