Determination of growth and glycerol production kinetics of a wine yeast strain Saccharomyces cerevisiae Kalecik 1 in different substrate media

• Published in: World journal of microbiology & biotechnology Vol. 21; no. 6-7; pp. 1303 - 1310
• Main Authors: YALCIN, Seda Karasu, ÖZBAS, Zekiye Yesim
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.10.2005; Springer Nature B.V
• Subjects: Biological and medical sciences; Biosynthesis; Biotechnology; Fermentation; Fundamental and applied biological sciences. Psychology; Glucose; Glycerol; Growth kinetics; Proteins; Saccharomyces cerevisiae; Sucrose; Vitaceae; Wines; Yeast; Yeasts; Osmolarity; Growth; Glycerol; Osmophily; growth kinetics; Wine yeast; Stress; Fungi; Substrate; osmotic stress; osmophilic yeast; Production; production kinetics; Ascomycetes; Kinetics; Saccharomyces cerevisiae; Thallophyta
• ISSN: 0959-3993; 1573-0972
• DOI: 10.1007/s11274-005-2634-9

Glycerol has been known as an important by-product of wine fermentations improving the sensory quality of wine. This study was carried out with an endogenic wine yeast strain Saccharomyces cerevisiae Kalecik 1. The kinetics of growth and glycerol biosynthesis were analysed at various initial concentrations of glucose, fructose, and sucrose in a batch system. Depending on the determined values of Monod constants, glucose (K^sub s^ = 28.09 g/l) was found as the most suitable substrate for the yeast growth. Initial glucose, fructose and sucrose concentrations necessary for maximum specific yeast growth rate were determined as 175 g, 100 l, and 200 g/l, respectively. The yeast produced glycerol at very high concentrations in fructose medium. Fructose was determined as the most suitable substrate for glycerol production while the strain showed low tendency to use it for growth. S. cerevisiae Kalecik 1 could not produce glycerol below 200 g/l initial sucrose concentration. When natural white grape juice was used as fermentation medium, maximum glycerol concentration and dry weight of the yeast were determined as 9.3 g/l and 11.8 g/l, respectively.[PUBLICATION ABSTRACT]