Simultaneous quantification six active compounds in rat plasma by UPLC–MS/MS and its application to a pharmacokinetic study of Pien-Tze-Huang

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 1061-1062; pp. 314 - 321
• Main Authors: Xu, Wen, Zhang, Yiping, Zhou, Caijie, Tai, Yanni, Zhang, Xiaoqing, Liu, Jie, Sha, Mei, Huang, Mingqing, Zhu, Yanlin, Peng, Jun, Lu, Jin-Jian
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2017
• Subjects: Animals; Chromatography, High Pressure Liquid - methods; Drugs, Chinese Herbal - analysis; Drugs, Chinese Herbal - chemistry; Drugs, Chinese Herbal - pharmacokinetics; Ginsenosides; Ginsenosides - blood; Ginsenosides - chemistry; Ginsenosides - pharmacokinetics; Linear Models; Male; Muscone; Pharmacokinetics; Pian-Tze-Huang; Rats; Rats, Sprague-Dawley; Reproducibility of Results; Sensitivity and Specificity; Tandem Mass Spectrometry - methods; UPLC–MS/MS; Pian-Tze-Huang; Muscone; Pharmacokinetics; UPLC–MS/MS; Ginsenosides
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2017.07.033

•Developing a UPLC-QqQ-MS/MS method to simultaneously determine five ginsenosides and muscone in rat plasma within 3.9min.•Pharmacokinetics profiles of six active compounds of Pien–Tze–Huang (PZH) after oral administration were obtained.•It is the first time to report the pharmacokinetics of PZH. Pien-Tze-Huang (PZH) is a popular traditional Chinese medicine (TCM) formula in China, but its pharmacokinetics has not been investigated yet. To better study the pharmacokinetic behaviors of PZH, an optimal ultra-performance liquid chromatography with triple quadrupole mass spectrometry (UPLC–MS/MS) method was developed for rapid quantification of six compounds (notoginsenoside R1, ginsenosides Re, Rg1, Rb1, Rd, and muscone) in rat plasma after oral administration of PZH. All analytes were extracted by protein precipitation with acetonitrile and separated on a Waters Acquity Cortecs C18 column within 3.9min, and detected by multiple-reaction monitoring in positive ion mode. This proposed method exhibited good linearity (r≥0.9932) with a lower quantification limits of 0.558–1.566ng/mL for all analytes. The intra- and inter-day precisions were within 8.24%, and the accuracy was within −10.05 to 9.87% for each analyte. The extraction recovery for each analyte ranged from 80.02 to 96.12%. This UPLC–MS/MS method was successfully applied to the pharmacokinetic study for PZH in rats.